1887

Abstract

Two strains of a Gram-negative, helical, haloalkaliphilic bacterium were isolated from Mono Lake (USA). Both strains were mesophilic and grew between 13 and 55 °C, with optimum growth at 35–45 °C. The optimum pH for growth was 9.5. Growth was observed at NaCl concentrations of 0.5–12 % (w/v), with optimum growth at 2 % NaCl. Both isolates were motile by means of bipolar tuft flagella, coccoid body-forming and strictly aerobic. It was concluded that they belong to the same species, based on DNA–DNA hybridization values (95 % DNA relatedness). DNA GC contents of the novel strains were 52.1 and 52.3 mol%. On the basis of 16S rRNA gene sequence similarity, both strains were shown to be related closely to the members of the genus (family , class ). Sequence similarity of strain v1c_Sn-red to the type strains of , , and was 95.0, 92.7, 91.8 and 91.8 %, respectively. Chemotaxonomic data [major ubiquinone, Q8; major fatty acids, C(n-7) and C] and physiological and biochemical tests supported the affiliation of the novel strains to the genus as members of a novel species, for which the name sp. nov. is proposed, with the type strain v1c_Sn-red (=LMG 24610=VKM 2416).

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2009-09-01
2020-01-22
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