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Volume 59, Issue 10

gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese Free

Abstract

The phylogenetic position and physiological characters of six hitherto-unknown lactic acid bacterial isolates, which form part of the surface microbiota of German red smear soft cheese, are reported. The coccoid cells are aerotolerant, Gram-positive, catalase-negative and non-motile. The cell-wall peptidoglycan contains alanine, glutamic acid, lysine and aspartic acid and is of the A4 type (-Lys–-Asp). The sequences of the 16S rRNA, and genes of the six isolates are identical and reveal that these isolates represent an independent lineage within the radiation of the family in the phylum . Their closest phylogenetic neighbour is the lactic acid bacterium M1590/94/2, with which they share 94.9 % 16S rRNA gene sequence similarity; representatives of other genera such as , and are more distantly related. DNA–DNA hybridization studies reveal that the six isolates are members of a single species, and this is confirmed by similarities in biochemical characteristics. The six isolates were assigned four different groups by Fourier-transform infrared and randomly amplified polymorphic DNA typing. Therefore, it is formally proposed that these isolates should be classified in a single novel species of a novel genus and be named gen. nov., sp. nov. The type strain of is WCC 4188 (=DSM 19936 =CCUG 55508).

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Keyword(s): FT-IR, Fourier-transform infrared and RAPD, randomly amplified polymorphic DNA
© SGM
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2009-10-01
2023-03-22
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Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese
Int J Syst Evol Microbiol 59, 2437 (2009); https://doi.org/10.1099/ijs.0.006601-0
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vol. , part 10, pp. 2437 - 2443

FT-IR-based similarity dendrogram for gen. nov., sp. nov. WCC 4188 and representatives of closely related genera.

FT-IR-based similarity dendrogram for the six strains of gen. nov., sp. nov., WCC 4185, WCC 4186, WCC 4187, WCC 4188 , WCC 4189 and WCC 4190, and comparison with M13 RAPD-fingerprint profiles.

Cellular fatty acid compositions of isolates WCC 4187, WCC 4188 , WCC 4189 and WCC 4190.

DNA–DNA relatedness between four strains of gen. nov., sp. nov.

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