1887

Abstract

During the course of a research project with free-living, nitrogen-fixing bacteria, we determined the 16S rRNA gene sequence of strains UQM 1685 and CIP 106281 and discovered that they were only 90.6–91.2 % similar to the sequences of strains of other species and subspecies. Moreover, the highest similarity to these sequences (99.7 %) corresponded to strains of (including ). Other diagnostic features confirmed that the two strains have the same origin but do not descend from the nomenclatural type. At the same time, LMG 2819 was characterized and it was found that its properties also do not agree with the original description of the species, although it can be considered a member of the genus. Further characterization, including chemotaxonomic and other phenotypic traits, allows us to propose (i) the identification of strains CIP 106281 and UQM 1685 as strains of and (ii) the designation of strain LMG 2819 (=CECT 7311) as the type strain of a novel species, sp. nov.

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2009-09-01
2019-10-15
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ARDRA profiles generated with I (a) and I (b). Lanes: 1, subsp. LMG 2817 ; 2, subsp. LMG 2818 ; 3, LMG 2819; 4, subsp. DSM 2329 ; 5, subsp. LMG 3899 ; 6, LMG 3912 ; 7, LMG 3977 ; 8, s NCIMB 9881; 9, NCIMB 9882; 10, NCIMB 11068; 11, s NCIMB 11069; 12, CIP 106281 ; 13, ACM 1685 ; 14, DSM 6462 ; M, DNA molecular weight marker XVII (Roche); M1, 20 bp DNA ladder (Takara).

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ARDRA dendrogram showing phylogenetic relationships between some members of the family . [PDF](38 KB)

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