1887

Abstract

A Gram-positive bacterial strain, designated YIM 56238, was isolated from plant roots (), and characterized by using a polyphasic approach. Strain YIM 56238 grew optimally at pH 7.0–8.0 and at 28 °C. Analysis of the 16S rRNA gene sequence of strain YIM 56238 indicated that it belongs to the genus . Chemotaxonomic data strongly supported the classification of this strain within the genus : the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H) (63.6 %) and MK-7(H) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C (30.95 %) and anteiso-C (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238 from recognized species of the genus . DNA–DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 56238 (=DSM 17945=KCTC 19156).

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2009-05-01
2022-01-22
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vol. , part 5, pp. 1070 - 1075

Scanning electron micrograph of cells of strain YIM 56238 grown on TSA for 3 days at 28 °C. Bar, 1 µm.



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