An extremely thermophilic, strictly anaerobic, facultatively chemolithoautotrophic bacterium designated strain DS was isolated from Treshchinnyi Spring, one of the hottest springs of the Uzon Caldera (Kamchatka, Russia). Cells of the novel organism were Gram-negative rods, about 1.0–1.2 μm long and 0.5 μm wide. The temperature range for growth was 52–82 °C, with an optimum at 75 °C. Growth was observed at pH 6.8–7.4, and the optimum pH was 7.0–7.2. Strain DS was able to grow lithoautotrophically with hydrogen in the presence of CO as a carbon source and thiosulfate or elemental sulfur as an electron acceptor. It also grew well with ethanol, fumarate, succinate or malate in the presence of thiosulfate. Yeast extract was not required for growth and did not stimulate growth. The genomic DNA G+C content was 35.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the novel organism was a member of the family . On the basis of phylogenetic and physiological considerations, it is proposed that strain DS represents a new genus and species, gen. nov., sp. nov. The type strain of is DS (=DSM 19393 =VKM B-2460).


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  1. Bonch-Osmolovskaya, E. A., Sokolova, T. G., Kostrikina, N. A. & Zavarzin, G. A.(1990).Desulfurella acetivorans gen. nov., sp. nov. – a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153, 151–155.[CrossRef] [Google Scholar]
  2. Garrity, G. M. & Holt, J. G.(2001). Phylum BIII. Thermodesulfobacteria phy. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, p. 389. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  3. Jeanthon, C., L'Haridon, S., Cueff, V., Banta, A., Reysenbach, A.-L. & Prieur, D.(2002).Thermodesulfobacterium hydrogeniphilum sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at Guaymas Basin, and emendation of the genus Thermodesulfobacterium. Int J Syst Evol Microbiol 52, 765–772.[CrossRef] [Google Scholar]
  4. Kashefi, K., Holmes, D. E., Reysenbach, A.-L. & Lovley, D. R.(2002). Use of Fe(III) as an electron acceptor to recover previously uncultured hyperthermophiles: isolation and characterization of Geothermobacterium ferrireducens gen. nov., sp. nov. Appl Environ Microbiol 68, 1735–1742.[CrossRef] [Google Scholar]
  5. L'Haridon, S., Reysenbach, A.-L., Tindall, B. J., Schönheit, P., Banta, A., Johnsen, U., Schumann, P., Gambacorta, A., Stackebrandt, E. & Jeanthon, C.(2006).Desulfurobacterium atlanticum sp. nov., Desulfurobacterium pacificum sp. nov. and Thermovibrio guaymasensis sp. nov., three thermophilic members of the Desulfurobacteriaceae fam. nov., a deep branching lineage within the Bacteria. Int J Syst Evol Microbiol 56, 2843–2852.[CrossRef] [Google Scholar]
  6. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  7. Marmur, J. & Doty, D.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  8. Moussard, H., L'Haridon, S., Tindall, B. J., Banta, A., Schumann, P., Stackebrandt, E., Reysenbach, A.-L. & Jeanthon, C.(2004).Thermodesulfatator indicus gen. nov., sp. nov., a novel thermophilic chemolithotrophic sulfate-reducing bacterium isolated from the Central Indian Ridge. Int J Syst Evol Microbiol 54, 227–233.[CrossRef] [Google Scholar]
  9. Nunoura, T., Oida, H., Misavuki, M. & Suzuki, Y.(2008).Thermosulfidibacter takaii gen. nov., sp. nov., a thermophilic, hydrogen-oxidizing, sulfur-reducing bacterium isolated from a deep-sea hydrothermal field in the Southern Okinawa Trough. Int J Syst Evol Microbiol 58, 659–665.[CrossRef] [Google Scholar]
  10. Pfennig, N. & Lippert, K. D.(1966). Über das Vitamin B12-Bedürfnis phototropher Schwefelbacterien. Arch Microbiol 55, 245–256 (in German). [Google Scholar]
  11. Rosanova, E. P. & Pivovarova, T. A.(1988). Reclassification of Desulfovibrio thermophilus (Rosanova and Khudyakova, 1974). Microbiology (English translation of Mikrobiologiia) 57, 102–106. [Google Scholar]
  12. Slobodkin, A. I., Tourova, T. P., Kuznetsov, B. B., Kostrikina, N. A., Chernyh, N. A. & Bonch-Osmolovskaya, E. A.(1999).Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium. Int J Syst Bacteriol 49, 1471–1478.[CrossRef] [Google Scholar]
  13. Sonne-Hansen, J. & Ahring, B. K.(1999).Thermodesulfobacterium hveragerdense sp. nov and Thermodesulfovibrio islandicus sp. nov., two thermophilic sulfate reducing bacteria isolated from a Icelandic hot spring. Syst Appl Microbiol 22, 559–564.[CrossRef] [Google Scholar]
  14. Trüper, H. G. & Schlegel, H. G.(1964). Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. Antonie van Leeuwenhoek 30, 225–238.[CrossRef] [Google Scholar]
  15. Vatsurina, A., Badrutdinova, D., Schumann, P., Spring, S. & Vainshtein, M.(2008).Desulfosporosinus hippei sp. nov., a mesophilic sulfate-reducing bacterium isolated from permafrost. Int J Syst Evol Microbiol 58, 1228–1232.[CrossRef] [Google Scholar]
  16. Wolin, E. A., Wolin, M. J. & Wolfe, R. S.(1963). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886. [Google Scholar]
  17. Zeikus, J. G., Dawson, M. A., Thompson, T. E., Ingvorsen, K. & Hatchikian, E. C.(1983). Microbial ecology of volcanic sulphidogenesis: isolation and characterization of Thermodesulfobacterium commune gen. nov and sp. nov. J Gen Microbiol 129, 1159–1169. [Google Scholar]

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vol. , part 5, pp. 1040 - 1044

Phylogenetic tree based on almost-complete 16S rRNA gene sequences and reconstructed using the program DNAPARS implemented in the PHYLIP package of the ARB software (version 03_08_22) showing the position of strain DS . [PDF](379 KB)


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