
Full text loading...
An extremely thermophilic, strictly anaerobic, facultatively chemolithoautotrophic bacterium designated strain DST was isolated from Treshchinnyi Spring, one of the hottest springs of the Uzon Caldera (Kamchatka, Russia). Cells of the novel organism were Gram-negative rods, about 1.0–1.2 μm long and 0.5 μm wide. The temperature range for growth was 52–82 °C, with an optimum at 75 °C. Growth was observed at pH 6.8–7.4, and the optimum pH was 7.0–7.2. Strain DST was able to grow lithoautotrophically with hydrogen in the presence of CO2 as a carbon source and thiosulfate or elemental sulfur as an electron acceptor. It also grew well with ethanol, fumarate, succinate or malate in the presence of thiosulfate. Yeast extract was not required for growth and did not stimulate growth. The genomic DNA G+C content was 35.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the novel organism was a member of the family Thermodesulfobacteriaceae. On the basis of phylogenetic and physiological considerations, it is proposed that strain DST represents a new genus and species, Caldimicrobium rimae gen. nov., sp. nov. The type strain of Caldimicrobium rimae is DST (=DSM 19393T =VKM B-2460T).
Article metrics loading...
Full text loading...
References
Data & Media loading...
Supplements
International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 5, pp. 1040 - 1044
Supplementary Fig. S1. Phylogenetic tree based on almost-complete 16S rRNA gene sequences and reconstructed using the program DNAPARS implemented in the PHYLIP package of the ARB software (version 03_08_22) showing the position of strain DS T. [PDF](379 KB)