A novel lactic acid bacterium, strain TMW 2.694, was isolated among other lactic acid bacteria from palm wine, an alcoholic beverage produced from the sap of various palm tree species. Strain TMW 2.694 is a Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming coccus, occurring in long chains. Phylogenetic analysis based on 16S rRNA gene sequencing positioned strain TMW 2.694 in a distinct line of descent within the genus , with the closest neighbours being JCM 6123 (98.7 % sequence similarity) and DSM 5577 (98.8 % sequence similarity). Comparative sequencing of the additional phylogenetic markers and confirmed the 16S rRNA gene tree topology. Genomic DNA–DNA similarities of strain TMW 2.694 to DSM 20202 and DSM 5577 were 45.1 and 17.7 %, respectively. The DNA G+C content is 36.4 mol%. Thus, we propose a novel species within the genus , with the name sp. nov. and the type strain TMW 2.694 (=DSM 21144 =LMG 24510).


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vol. , part 5, pp. 943 – 947

Maximum-likelihood tree derived from 16S rRNA gene sequence analysis, showing the relationship of TMW 2.694 to other members of the genus

Maximum-parsimony tree derived from 16S rRNA gene sequence analysis, showing the relationship of TMW 2.694 to other members of the genus

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