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Volume 59, Issue 9

sp. nov., a dehalogenating bacterium in the from the marine sponge Free

Abstract

A reductively dehalogenating, strictly anaerobic, sulfate-reducing bacterium, designated strain AA1, was isolated from the marine sponge collected in the Mediterranean Sea and was characterized phenotypically and phylogenetically. Cells of strain AA1 were Gram-negative, short, curved rods. Growth of strain AA1 was observed between 20 and 37 °C (optimally at 28 °C) at pH 7–8. NaCl was required for growth; optimum growth occurred in the presence of 25 g NaCl l. Growth occurred with lactate, propionate, pyruvate, succinate, benzoate, glucose and sodium citrate as electron donors and carbon sources and either sulfate or 2-bromophenol as electron acceptors, but not with acetate or butyrate. Strain AA1 was able to dehalogenate several different bromophenols, and 2- and 3-iodophenol, but not monochlorinated or fluorinated phenols. Lactate, pyruvate, fumarate and malate were not utilized without an electron acceptor. The G+C content of the genomic DNA was 58.5 mol%. The predominant cellular fatty acids were C, iso-C, C 3-OH, anteiso-C, C, C 7 and C 7. Phylogenetic analysis based on 16S rRNA gene sequence comparisons placed the novel strain within the class . Strain AA1 was related most closely to the type strains of (96 % 16S rRNA gene sequence similarity), (95 %) and (95 %). Based on its phenotypic, physiological and phylogenetic characteristics, strain AA1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AA1 (=DSM 17682 =ATCC BAA-1256).

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Keyword(s): 2BP, 2-bromophenol , APS, adenosine 5′-phosphosulfate , DGGE, denaturing gradient gel electrophoresis , T-RFLP, terminal restriction fragment length polymorphism and TRF, terminal restriction fragment
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2009-09-01
2024-04-26
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Desulfoluna spongiiphila sp. nov., a dehalogenating bacterium in the Desulfobacteraceae from the marine sponge Aplysina aerophoba
Int J Syst Evol Microbiol 59, 2133 (2009); https://doi.org/10.1099/ijs.0.005884-0
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T-RFLP electropherogram analysis of a sponge enrichment on bromophenols compared to a pure culture of strain AA1 .

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Fatty acid compositions of the sponge-associated bacterium strain AA1 and type strains of related and species. [PDF](60 KB)

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