Phylogenetic analysis and species identification of members of the genus were carried out using partial sequence comparison of the 16S rRNA gene (1468–1478 bp), , encoding the subunit of RNA polymerase (659–680 bp), , encoding the manganese-dependent superoxide dismutase (435–462 bp), , encoding the 60 kDa heat-shock protein (757 bp), and , encoding the Β subunit of DNA gyrase (458–461 bp). For the first time, most species within the genus were represented in the study (65 strains, representing 58 species and nine subspecies). Phylogenies inferred from , , and sequence comparisons were more discriminative than those inferred from 16S rRNA gene sequence comparison, and showed common clusters. The minimal interspecies divergence was 0.3, 2.7, 0, 2.5 and 3.4 % for the 16S rRNA gene, , , and , respectively. In general, partial gene sequence comparison represented the best tool for identifying species and subspecies and for phylogenetic analysis.


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vol. , part 9, pp. 2317 - 2322

Strains used in this study and nucleotide sequence accession numbers.

Primers for PCR and sequencing of and genes.

Phylogenetic tree of members of the genus inferred from comparison of 16S rRNA gene sequences using the neighbour-joining method.

Phylogenetic trees of members of the genus inferred from comparison of partial , and gene sequences using the neighbour-joining method.

Schematic representation of similarity between different species for each gene studied.

[PDF file of Supplementary Tables and Figures](233 KB)

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