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Phylogenetic analysis and species identification of members of the genus Streptococcus were carried out using partial sequence comparison of the 16S rRNA gene (1468–1478 bp), rpoB, encoding the β subunit of RNA polymerase (659–680 bp), sodA, encoding the manganese-dependent superoxide dismutase (435–462 bp), groEL, encoding the 60 kDa heat-shock protein (757 bp), and gyrB, encoding the Β subunit of DNA gyrase (458–461 bp). For the first time, most species within the genus Streptococcus were represented in the study (65 strains, representing 58 species and nine subspecies). Phylogenies inferred from rpoB, sodA, gyrB and groEL sequence comparisons were more discriminative than those inferred from 16S rRNA gene sequence comparison, and showed common clusters. The minimal interspecies divergence was 0.3, 2.7, 0, 2.5 and 3.4 % for the 16S rRNA gene, rpoB, sodA, gyrB and groEL, respectively. In general, groEL partial gene sequence comparison represented the best tool for identifying species and subspecies and for phylogenetic analysis.
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 9, pp. 2317 - 2322
Supplementary Table S1. Strains used in this study and nucleotide sequence accession numbers.
Supplementary Table S2. Primers for PCR and sequencing of groEL and gyrB genes.
Supplementary Fig. S1. Phylogenetic tree of members of the genus Streptococcus inferred from comparison of 16S rRNA gene sequences using the neighbour-joining method.
Supplementary Fig. S2. Phylogenetic trees of members of the genus Streptococcus inferred from comparison of partial sodA , rpoB and gyrB gene sequences using the neighbour-joining method.
Supplementary Fig. S3. Schematic representation of similarity between different species for each gene studied.
[PDF file of Supplementary Tables and Figures](233 KB)