A phenol-degrading actinomycete, strain NP8-5, was isolated from a polluted stream in Gumi, Korea. The strain was aerobic, Gram-positive-staining, non-motile and non-spore-forming, displayed a rod–coccus growth cycle, exhibited white opaque colonies on complex media and showed chemotaxonomic markers that were consistent with its classification in the genus . Phylogenetic analysis, based on 16S rRNA gene sequencing, also showed that strain NP8-5 belonged to the genus , sharing the highest levels of sequence similarity with IFM 10211, DSM 44015 and NCIMB 13802 (96.4, 96.0 and 95.9 %, respectively) and forming a separate lineage within this genus. Combined phylogenetic and phenotypic data supported the conclusion that strain NP8-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NP8-5 (=KCTC 19360 =DSM 45133).


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  1. Arenskötter, M., Bröker, D. & Steinbüchel, A.(2004). Biology of the metabolically diverse genus Gordonia. Appl Environ Microbiol 70, 3195–3204.[CrossRef] [Google Scholar]
  2. Arenskötter, M., Linos, A., Schumann, P., Kroppenstedt, R. M. & Steinbüchel, A.(2005).Gordonia nitida Yoon et al. 2000 is a later synonym of Gordonia alkanivorans Kummer et al. 1999. Int J Syst Evol Microbiol 55, 695–697.[CrossRef] [Google Scholar]
  3. Bates, R. G. & Bower, V. E.(1956). Alkaline solutions for pH control. Anal Chem 28, 1322–1324.[CrossRef] [Google Scholar]
  4. Bendinger, B., Rainey, F. A., Kroppenstedt, R. M., Moormann, M. & Klatte, S.(1995).Gordona hydrophobica sp. nov., isolated from biofilters for waste gas treatment. Int J Syst Bacteriol 45, 544–548.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors)(1994).Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  9. Goodfellow, M. & Maldonado, L. A.(2006). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 843–888. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  10. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  11. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  12. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  13. Kim, K. K., Lee, C. S., Kroppenstedt, R. M., Stackebrandt, E. & Lee, S. T.(2003).Gordonia sihwensis sp. nov., a novel nitrate-reducing bacterium from a wastewater-treatment bioreactor. Int J Syst Evol Microbiol 53, 1427–1433.[CrossRef] [Google Scholar]
  14. Klatte, S., Rainey, F. A. & Kroppenstedt, R. M.(1994). Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol 44, 769–773.[CrossRef] [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  16. Linos, A., Steinbüchel, A., Spröer, C. & Kroppenstedt, R. M.(1999).Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre. Int J Syst Bacteriol 49, 1785–1791.[CrossRef] [Google Scholar]
  17. Linos, A., Berekaa, M. M., Steinbüchel, A., Kim, K. K., Spröer, C. & Kroppenstedt, R. M.(2002).Gordonia westfalica sp. nov., a novel rubber-degrading actinomycete. Int J Syst Evol Microbiol 52, 1133–1139.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Patel, P. V., Alshamaony, L. & Goodfellow, M.(1977). Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27, 104–117.[CrossRef] [Google Scholar]
  19. Pascual, C., Lawson, P. A., Farrow, J. A. E., Gimenez, M. N. & Collins, M. D.(1995). Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int J Syst Bacteriol 45, 724–728.[CrossRef] [Google Scholar]
  20. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a district actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  21. Saitou, N. & Nei, M.(1987). The neighbor-joining method; a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  22. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H.(1996). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69. [Google Scholar]
  24. Soddell, J. A., Stainsby, F. M., Eales, K. L., Kroppenstedt, R. M., Seviour, R. J. & Goodfellow, M.(2006).Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 56, 739–744.[CrossRef] [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  26. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  27. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  29. Uchida, K., Kudo, T., Suzuki, K. & Nagase, T.(1999). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef] [Google Scholar]

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vol. , part 8, pp. 1992 - 1996

Chemotaxonomic characteristics of strain NP8-5 and closely related type strains. [PDF](59 KB)

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