1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 8

gen. nov., sp. nov., a moderately thermophilic member of the family isolated from a coastal hot spring Free

Abstract

A yellow-pigmented, Gram-staining-negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, CC-MHSW-2, was isolated from a coastal hot spring of Green Island (Lutao), located off Taituang, Taiwan. The 16S rRNA gene sequence analysis demonstrated that it shared <93.2 % sequence similarity with species. The organism was unable to produce acid from carbohydrates, but it could utilize a number of organic acids and amino acids. Menaquinone 6 (MK-6) was the major respiratory quinone and iso-C, iso-C 3-OH and summed feature 3 (comprising C 7 and/or C 6) were the predominant fatty acids. This fatty acid profile distinguished the isolate from members of the genera , , , and . The DNA G+C content of strain CC-MHSW-2 was 39.7±1 mol%. On the basis of the 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain CC-MHSW-2 represents a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CC-MHSW-2 (=KCTC 22538 =JCM 15069).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.005256-0
2009-08-01
2019-10-13

  • From This Site

    /content/journal/ijsem/10.1099/ijs.0.005256-0
    dcterms_title,dcterms_subject,pub_keyword
    10
    5
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/8/2069.html?itemId=/content/journal/ijsem/10.1099/ijs.0.005256-0&mimeType=html&fmt=ahah

References

  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa com. nov. Int J Syst Evol Microbiol 50, 1861–1868.
     [Google Scholar]
  3. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  4. Chung, Y. C., Kobayashi, T., Kanai, H., Akiba, T. & Kudo, T. ( 1995; ). Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococccus profundus DT5432. Appl Environ Microbiol 61, 1502–1506.
     [Google Scholar]
  5. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  8. Johansen, J. E., Nielsen, P. & Sjøholm, C. ( 1999; ). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  11. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
     [Google Scholar]
  12. Lee, S. D. ( 2007; ). Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. Int J Syst Evol Microbiol 57, 764–769.[CrossRef]
    [Google Scholar]
  13. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
     [Google Scholar]
  14. Liu, Z.-P., Wang, B.-J., Dai, X., Liu, X.-Y. & Liu, S.-J. ( 2006; ). Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea. Int J Syst Evol Microbiol 56, 2825–2829.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Miller, P. H., Wiggs, L. S. & Miller, J. M. ( 1995; ). Evaluation of AnaeroGen system for growth of anaerobic bacteria. J Clin Microbiol 33, 2388–2391.
     [Google Scholar]
  17. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  18. Nedashkovskaya, O. I., Suzuki, M., Lysenko, A. M., Snauwaert, C., Vancanneyt, M., Swings, J., Vysotskii, M. V. & Mikhailov, V. V. ( 2004; ). Cellulophaga pacifica sp. nov. Int J Syst Evol Microbiol 54, 609–613.[CrossRef]
    [Google Scholar]
  19. Nedashkovskaya, O. I., Vancanneyt, M., Dawyndt, P., Engelbeen, K., Vandemeulebroecke, K., Cleenwerck, I., Hoste, B., Mergaert, J., Tan, T. L. & other authors ( 2005; ). Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov. Int J Syst Evol Microbiol 55, 1033–1038.[CrossRef]
    [Google Scholar]
  20. Nedashkovskaya, O. I., Vancanneyt, M., Christiaens, L., Kalinovskaya, N. I., Mikhailov, V. V. & Swings, J. ( 2006; ). Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina. Int J Syst Evol Microbiol 56, 2037–2041.[CrossRef]
    [Google Scholar]
  21. Pinhassi, J., Bowman, J. P., Nedashkovskaya, O. I., Lekunberri, I., Gomez-Consarnau, L. & Pedrós-Alió, C. ( 2006; ). Leeuwenhoekiella blandensis sp. nov., a genome-sequenced marine member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 56, 1489–1493.[CrossRef]
    [Google Scholar]
  22. Reichenbach, H. ( 1992; ). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  23. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  24. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  25. Yoon, J.-H., Kang, S.-J., Jung, S.-Y., Oh, H. W. & Oh, T.-K. ( 2006; ). Gaetbulimicrobium brevivitae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 56, 115–119.[CrossRef]
    [Google Scholar]
  26. Young, C.-C., Kämpfer, P., Shen, F.-T., Lai, W.-A. & Arun, A. B. ( 2005; ). Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55, 423–426.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.005256-0
Loading
Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring
Int J Syst Evol Microbiol 59, 2069 (2009); https://doi.org/10.1099/ijs.0.005256-0
/content/journal/ijsem/10.1099/ijs.0.005256-0
/content/journal/ijsem/10.1099/ijs.0.005256-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error