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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 9

gen. nov., sp. nov., isolated from soil Free

Abstract

A yellow-pigmented, Gram-negative, aerobic, rod-shaped bacterium, strain KIS3-4, was isolated from soil collected on Daechung Island in the West Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence placed strain KIS3-4 in a distinct lineage in the family . Strain KIS3-4 shared 87.3–93.7 % sequence similarity with members of the family , and was related most closely to the genera and . In its biochemical characteristics, strain KIS3-4 was clearly separable from other genera within the family on the basis of the hydrolysis of cellulose and urea, high G+C content (64 mol%) and fatty acid profile. Major fatty acids (>10 % of the total fatty acids) were iso-C 9 (32.8 %), iso-C (18.0 %) and iso-C (12.7 %). Q-8 was the predominant respiratory quinone. Phosphatidylethanolamine and several unidentified aminophospholipids and phospholipids were present. Based on its unique phenotypic, genotypic and phylogenetic features, strain KIS3-4 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is KIS3-4 (=KACC 12734 =JCM 15422).

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2009-09-01
2025-04-23
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Rudaea cellulosilytica gen. nov., sp. nov., isolated from soil
Int J Syst Evol Microbiol 59, 2308 (2009); https://doi.org/10.1099/ijs.0.005165-0
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Transmission electron micrograph of cells of strain KIS3-4 . Bar, 1 µm.

IMAGE

Polar lipid profiles of strain KIS3-4 (a) and DSM 17203 (b) after separation by two-dimensional TLC. APL1–4, Unidentified aminophospholipids; L, unidentified polar lipid; PE, phosphatidylethanolamine; PL1–4, unidentified phospholipids.

IMAGE

Polar lipid profiles of strain KIS3-4 (a) and DSM 17203 (b) after separation by two-dimensional TLC. APL1–4, Unidentified aminophospholipids; L, unidentified polar lipid; PE, phosphatidylethanolamine; PL1–4, unidentified phospholipids.

IMAGE

Fatty acid comparisons among strain KIS3-4 and related members of the family . [PDF](73 KB)

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