A novel, xylanolytic, anaerobic, moderately thermophilic bacterium, strain PML14, was isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China. The strain was a Gram-positive, spore-forming and motile rod. Growth of the novel strain was observed at 25–67 °C (optimum 60 °C) and pH 5.8–9.3 (optimum pH 8.5). Strain PML14 grew on a number of carbohydrates, including xylan, xylose, glucose and cellobiose, and a variety of proteinaceous compounds, including peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, -cysteine, -serine, -lysine, -glycine, -threonine, -methionine and pyruvate. The fermentation products from glucose included acetate, ethanol, butyrate, hydrogen and carbon dioxide. Propionate was produced from xylan in addition to other compounds. Fe(III), 9,10-anthraquinone 2,6-disulfonate and thiosulfate were reduced with peptone as the electron donor. NH was produced. Indole was not produced. Gelatin was not hydrolysed. The DNA G+C content of strain PML14 was 36.2±0.8 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PML14 was related to the members of cluster XII of the clostridia, most closely to SB91 with 93.8 % 16S rRNA gene sequence similarity. On the basis of polyphasic evidence from this study, it is suggested that strain PML14 (=CGMCC 1.5080=JCM 15035) represents a novel species of the genus , for which the name sp. nov., is proposed. An emended description of the genus is also provided.


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