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Abstract

A novel, xylanolytic, anaerobic, moderately thermophilic bacterium, strain PML14, was isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China. The strain was a Gram-positive, spore-forming and motile rod. Growth of the novel strain was observed at 25–67 °C (optimum 60 °C) and pH 5.8–9.3 (optimum pH 8.5). Strain PML14 grew on a number of carbohydrates, including xylan, xylose, glucose and cellobiose, and a variety of proteinaceous compounds, including peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, -cysteine, -serine, -lysine, -glycine, -threonine, -methionine and pyruvate. The fermentation products from glucose included acetate, ethanol, butyrate, hydrogen and carbon dioxide. Propionate was produced from xylan in addition to other compounds. Fe(III), 9,10-anthraquinone 2,6-disulfonate and thiosulfate were reduced with peptone as the electron donor. NH was produced. Indole was not produced. Gelatin was not hydrolysed. The DNA G+C content of strain PML14 was 36.2±0.8 mol% ( ). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PML14 was related to the members of cluster XII of the clostridia, most closely to SB91 with 93.8 % 16S rRNA gene sequence similarity. On the basis of polyphasic evidence from this study, it is suggested that strain PML14 (=CGMCC 1.5080=JCM 15035) represents a novel species of the genus , for which the name sp. nov., is proposed. An emended description of the genus is also provided.

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2009-11-01
2020-01-22
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References

  1. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Chen, S. & Dong, X. ( 2004; ). Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol 54, 2257–2262.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combination. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  4. Das, D. & Veziroglu, T. N. ( 2001; ). Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy 26, 13–28.[CrossRef]
    [Google Scholar]
  5. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  6. Hungate, R. E. ( 1969; ). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  7. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  8. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  9. Nandi, R. & Sengupta, S. ( 1998; ). Microbial production of hydrogen: an overview. Crit Rev Microbiol 24, 61–84.[CrossRef]
    [Google Scholar]
  10. Niu, L., Song, L. & Dong, X. ( 2008; ). Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium. Int J Syst Evol Microbiol 58, 12–16.[CrossRef]
    [Google Scholar]
  11. Schnürer, A., Schink, B. & Svensson, B. H. ( 1996; ). Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogentrophic methanogenic bacterium. Int J Syst Bacteriol 46, 1145–1152.[CrossRef]
    [Google Scholar]
  12. Slobodkin, A. I., Tourova, T. P., Kostrikina, N. A., Lysenko, A. M., German, K. E., Bonch-Osmolovskaya, E. A. & Birkeland, N.-K. ( 2006; ). Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales. Int J Syst Evol Microbiol 56, 369–372.[CrossRef]
    [Google Scholar]
  13. Zehnder, A. J. B. ( 1978; ). Ecology of methane formation. In Water Pollution Microbiology, pp. 349–376. Edited by R. Mitchell. New York: Wiley.
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