1887

Abstract

The taxonomic position of a group of four strains, isolated from the phyllosphere of grasses, within the species was investigated. The isolates formed a separate cluster through ribotyping and MALDI-TOF MS, which could be clearly differentiated from the type strain of . The differences found between the patterns of the type strain of and the novel isolates were more distinct than those between the type strain and recognized species of the genus , which were phylogenetically related by 16S rRNA gene sequence analysis. Physiological characterization also revealed significant differences between the novel grass isolates and the type strain of . Siderotyping of the pyoverdines revealed identical pyoverdine-isoelectrofocusing patterns for the novel isolates and the type strain of . However, pyoverdine-mediated Fe cross uptake studies indicated differences in the siderotype. In contrast, phylogenetic analysis based on 16S rRNA gene sequence analysis and DNA–DNA hybridization studies (reassociation value 76.4 %) supported the affiliation of the novel isolates to the species . As a consequence of these observations, the splitting of the species into two novel subspecies subsp. subsp. nov. (type strain CFML 96-198=CIP 105541=DSM 17516) and subsp. subsp. nov. (type strain P 515/12=DSM 14938=LMG 21467) is proposed.

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2009-06-01
2019-10-22
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References

  1. Behrendt, U., Ulrich, A., Schumann, P., Erler, W., Burghardt, J. & Seyfarth, W. ( 1999; ). A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov. Int J Syst Bacteriol 49, 297–308.[CrossRef]
    [Google Scholar]
  2. Behrendt, U., Ulrich, A. & Schumann, P. ( 2003; ). Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int J Syst Evol Microbiol 53, 1461–1469.[CrossRef]
    [Google Scholar]
  3. Behrendt, U., Ulrich, A., Schumann, P., Meyer, J.-M. & Spröer, C. ( 2007; ). Pseudomonas lurida sp. nov., a fluorescent species associated with the phyllosphere of grasses. Int J Syst Evol Microbiol 57, 979–985.[CrossRef]
    [Google Scholar]
  4. Behrendt, U., Ulrich, A. & Schumann, P. ( 2008; ). Chryseobacterium gregarium sp. nov., isolated from decaying plant material. Int J Syst Evol Microbiol 58, 1069–1074.[CrossRef]
    [Google Scholar]
  5. Clark, L. L., Dajcs, J. J., McLean, C. H., Bartell, J. G. & Stroman, D. W. ( 2006; ). Pseudomonas otitidis sp. nov., isolated from patients with otic infections. Int J Syst Evol Microbiol 56, 709–714.[CrossRef]
    [Google Scholar]
  6. Conway, G. C., Smole, S. C., Sarracino, D. A., Arbeit, R. D. & Leopold, P. E. ( 2001; ). Phyloproteomics: species identification of Enterobacteriaceae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Mol Microbiol Biotechnol 3, 103–112.
    [Google Scholar]
  7. Dabboussi, F., Hamze, M., Elomari, M., Verhille, S., Baida, N., Izard, D. & Leclerc, H. ( 1998; ). A numerical study of fluorescent Pseudomonas strains isolated from three Lebanese spring waters. J Eur Hydrol 28, 325–338.
    [Google Scholar]
  8. Dabboussi, F., Hamze, M., Elomari, M., Verhille, S., Baida, N., Izard, D. & Leclerc, H. ( 1999; ). Taxonomic study of bacteria isolated from Lebanese spring waters: proposal for Pseudomonas cedrella sp. nov. and P. orientalis sp. nov. Res Microbiol 150, 303–316.[CrossRef]
    [Google Scholar]
  9. Fenselau, C. & Demirev, P. A. ( 2001; ). Characterization of intact microorganisms by MALDI mass spectrometry. Mass Spectrom Rev 20, 157–171.[CrossRef]
    [Google Scholar]
  10. Fuchs, R., Schäfer, M., Geoffroy, V. & Meyer, J.-M. ( 2001; ). Siderotyping – a powerful tool for the characterization of pyoverdines. Curr Top Med Chem 1, 31–57.[CrossRef]
    [Google Scholar]
  11. Gardan, L., Shafik, H., Belouin, S., Broch, R., Grimont, F. & Grimont, P. A. D. ( 1999; ). DNA relatedness among the pathovars of Pseudomonas syringae and description of Pseudomonas tremae sp. nov. and Pseudomonas cannabina sp. nov. (ex Sutic and Dowson 1959). Int J Syst Bacteriol 49, 469–478.[CrossRef]
    [Google Scholar]
  12. Hildebrand, D. C., Schroth, M. N. & Sands, D. C. ( 1994; ). Pseudomonas. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, pp. 60–80. Edited by N. W. Schaad. St Paul, MN: American Phytopathological Society.
  13. Lapage, S. P., Sneath, P. H. A., Lessel, E. F., Skerman, V. B. D., Seeliger, H. P. R. & Clark, W. A. (editors) ( 1992; ). International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code. Washington, DC: American Society for Microbiology.
  14. Meyer, J.-M. & Geoffroy, V. ( 2004; ). Environmental fluorescent Pseudomonas and pyoverdine diversity: how siderophores could help microbiologists in bacterial identification and taxonomy. In Iron Transport in Bacteria, pp. 451–468. Edited by J. H. Crosa, A. R. Mey & S. M. Payne. Washington, DC: American Society for Microbiology.
  15. Meyer, J.-M., Stintzi, A., Coulanges, V., Shivaji, S., Voss, J. A., Taraz, K. & Budzikiewicz, H. ( 1998; ). Siderotyping of fluorescent pseudomonads: characterization of pyoverdines of Pseudomonas fluorescens and Pseudomonas putida strains from Antarctica. Microbiology 144, 3119–3126.[CrossRef]
    [Google Scholar]
  16. Meyer, J.-M., Geoffroy, V. A., Baida, N., Gardan, L., Izard, D., Lemanceau, P., Achouak, W. & Palleroni, N. J. ( 2002; ). Siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads. Appl Environ Microbiol 68, 2745–2753.[CrossRef]
    [Google Scholar]
  17. Meyer, J.-M., Gruffaz, C., Tulkki, T. & Izard, D. ( 2007; ). Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Pseudomonas putida. Int J Syst Evol Microbiol 57, 2543–2556.[CrossRef]
    [Google Scholar]
  18. Peix, A., Valverde, A., Rivas, R., Igual, J. M., Ramírez-Bahena, M. H., Mateos, P. F., Santa-Regina, I., Rodríguez-Barrueco, C., Martínez-Molina, E. & Velázquez, E. ( 2007; ). Reclassification of Pseudomonas aurantiaca as a synonym of Pseudomonas chlororaphis and proposal of three subspecies, P. chlororaphis subsp. chlororaphis subsp. nov., P. chlororaphis subsp. aureofaciens subsp. nov., comb. nov. and P. chlororaphis subsp. aurantiaca subsp. nov., comb. nov. Int J Syst Evol Microbiol 57, 1286–1290.[CrossRef]
    [Google Scholar]
  19. Rudolph, K., Roy, M. A., Sasser, M., Stead, D. E., Davis, D. E., Swings, M. & Gosselé, F. ( 1990; ). Isolation of bacteria. In Methods in Phytobacteriology, pp. 43–86. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  20. Sikorski, J., Stackebrandt, E. & Wackernagel, W. ( 2001; ). Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 51, 1549–1555.
    [Google Scholar]
  21. Sikorski, J., Lalucat, J. & Wackernagel, W. ( 2005; ). Genomovars 11 to 18 of Pseudomonas stutzeri, identified among isolates from soil and marine sediment. Int J Syst Evol Microbiol 55, 1767–1770.[CrossRef]
    [Google Scholar]
  22. Stackebrandt, E., Päuker, O. & Erhard, M. ( 2005; ). Grouping myxococci (Corallococcus) strains by matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry: comparison with gene sequence phylogenies. Curr Microbiol 50, 71–77.[CrossRef]
    [Google Scholar]
  23. Sutra, L., Siverio, F., Lopez, M. M., Hunault, G., Bollet, C. & Gardan, L. ( 1997; ). Taxonomy of Pseudomonas strains isolated from tomato pith necrosis: emended description of Pseudomonas corrugata and proposal of three unnamed fluorescent Pseudomonas genomospecies. Int J Syst Bacteriol 47, 1020–1033.[CrossRef]
    [Google Scholar]
  24. Tóth, E. M., Schumann, P., Borsodi, A. K., Kéki, Z., Kovács, A. L. & Márialigeti, K. ( 2008; ). Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). Int J Syst Evol Microbiol 58, 976–981.[CrossRef]
    [Google Scholar]
  25. Valentine, N., Wunschel, S., Wunschel, D., Petersen, C. & Wahl, K. ( 2005; ). Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry. Appl Environ Microbiol 71, 58–64.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
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Riboprint patterns of RI-restricted DNA of the investigated grass isolates, the type strain of and highly related species as determined by 16S rRNA gene analysis. [ PDF] 46 KB

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Differential physiological characteristics of subsp. , subsp. and phylogenetically related species. [ PDF] 114 KB

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