The taxonomic position of a group of four strains, isolated from the phyllosphere of grasses, within the species was investigated. The isolates formed a separate cluster through ribotyping and MALDI-TOF MS, which could be clearly differentiated from the type strain of . The differences found between the patterns of the type strain of and the novel isolates were more distinct than those between the type strain and recognized species of the genus , which were phylogenetically related by 16S rRNA gene sequence analysis. Physiological characterization also revealed significant differences between the novel grass isolates and the type strain of . Siderotyping of the pyoverdines revealed identical pyoverdine-isoelectrofocusing patterns for the novel isolates and the type strain of . However, pyoverdine-mediated Fe cross uptake studies indicated differences in the siderotype. In contrast, phylogenetic analysis based on 16S rRNA gene sequence analysis and DNA–DNA hybridization studies (reassociation value 76.4 %) supported the affiliation of the novel isolates to the species . As a consequence of these observations, the splitting of the species into two novel subspecies subsp. subsp. nov. (type strain CFML 96-198=CIP 105541=DSM 17516) and subsp. subsp. nov. (type strain P 515/12=DSM 14938=LMG 21467) is proposed.


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Riboprint patterns of RI-restricted DNA of the investigated grass isolates, the type strain of and highly related species as determined by 16S rRNA gene analysis. [ PDF] 46 KB


Differential physiological characteristics of subsp. , subsp. and phylogenetically related species. [ PDF] 114 KB


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