1887

Abstract

A non-motile, coccobacilli-shaped, pale-pink-pigmented bacterium, designated strain CW67, was isolated from a water-cooling system in Gwangyang, Republic of Korea. Cells were found to be Gram-negative, catalase-positive and oxidase-positive, the major fatty acids were C 7 (43.6 %) and C (15.8 %), the predominant respiratory lipoquinone was Q-10 and the DNA G+C content was 69.5 mol%. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain CW67 forms an evolutionary lineage within the radiation of the genus and that its closest relative is subsp. MDA5605 (94.7 % sequence similarity). Evidence from this polyphasic study showed that strain CW67 could not be assigned to any recognized species. It therefore represents a novel species, for which the name sp. nov. is proposed, with CW67 (=KCTC 22211 =JCM 15073) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004812-0
2009-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/7/1630.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004812-0&mimeType=html&fmt=ahah

References

  1. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M.(1966). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496. [Google Scholar]
  3. Bibashi, E., Sofianou, D., Kontopoulou, K., Mitsopoulos, E. & Kokolina, E.(2000). Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 38, 456–457. [Google Scholar]
  4. Christensen, W. B.(1946). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466. [Google Scholar]
  5. Chun, J. & Goodfellow, M.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef] [Google Scholar]
  6. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J.(2000).Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913. [Google Scholar]
  7. CLSI(2003).Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical Laboratory Standards Institute.
  8. Collins, M. D.(1985). Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–288. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  9. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  10. Eiler, A. & Bertilsson, S.(2004). Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ Microbiol 6, 1228–1243.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  12. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef] [Google Scholar]
  13. Furuhata, K., Goto, K., Kato, Y., Saitou, K., Sugiyama, J., Hara, M., Yoshida, S. & Fukuyama, M.(2007). Characteristics of a pink-pigmented bacterium isolated from biofilm in a cooling tower in Tokyo, Japan. Microbiol Immunol 51, 637–641.[CrossRef] [Google Scholar]
  14. Gallego, V., Sánchez-Porro, C., García, M. T. & Ventosa, A.(2006).Roseomonas aquatica sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 56, 2291–2295.[CrossRef] [Google Scholar]
  15. Han, X. Y., Pham, A. S., Tarrand, J. J., Rolston, K. V., Helsel, L. O. & Levett, P. N.(2003). Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp. nov. and Roseomonas gilardii subsp. rosea subsp. nov. Am J Clin Pathol 120, 256–264.[CrossRef] [Google Scholar]
  16. Helsel, L. O., Hollis, D. G., Steigerwalt, A. G. & Levett, P. N.(2006). Reclassification of Roseomonas fauriae Rihs et al. 1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Int J Syst Evol Microbiol 56, 2753–2755.[CrossRef] [Google Scholar]
  17. Jiang, C.-Y., Dai, X., Wang, B.-J., Zhou, Y.-G. & Liu, S.-J.(2006).Roseomonas lacus sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56, 25–28.[CrossRef] [Google Scholar]
  18. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  19. Kovács, N.(1956). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703 [Google Scholar]
  20. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  21. MIDI(1999).Sherlock Microbial Identification System Operating Manual, version 3.0. Newark, DE: MIDI, Inc.
  22. Richard, C. & Kiredjian, M.(1995).Laboratory Methods for the Identification of Strictly Aerobic Gram-Negative Bacilli. Paris: Institut Pasteur.
  23. Rihs, J. D., Brenner, D. J., Weaver, R. E., Steigerwalt, A. G., Hollis, D. G. & Yu, V. L.(1993).Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 31, 3275–3283. [Google Scholar]
  24. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  25. Sandoe, J. A. T., Malnick, H. & Loudon, K. W.(1997). A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 35, 2150–2152. [Google Scholar]
  26. Satomi, M., Vogel, B. F., Venkateswaran, K. & Gram, L.(2007). Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. Int J Syst Evol Microbiol 57, 347–352.[CrossRef] [Google Scholar]
  27. September, S. M., Brozel, V. S. & Venter, S. N.(2004). Diversity of nontuberculoid Mycobacterium species in biofilms of urban and semiurban drinking water distribution systems. Appl Environ Microbiol 70, 7571–7573.[CrossRef] [Google Scholar]
  28. Sfanos, K., Harmody, D., Dang, P., Ledger, A., Pomponi, S., McCarthy, P. & Lopez, J.(2005). A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates. Syst Appl Microbiol 28, 242–264.[CrossRef] [Google Scholar]
  29. Skerman, V. B. D.(1967).A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  30. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  31. Subudhi, C. P. K., Adedeji, A., Kaufmann, M. E., Lucas, G. S. & Kerr, J. R.(2001). Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. Clin Microbiol Infect 7, 573–575.[CrossRef] [Google Scholar]
  32. Swofford, D. L.(1998). Phylogenetic analysis using parsimony (paup), version 4. Sunderland, MA: Sinauer Associates.
  33. Yoo, S. H., Weon, H. Y., Noh, H. J., Hong, S. B., Lee, C. M., Kim, B. Y., Kwon, S. W. & Go, S. J.(2008).Roseomonas aerilata sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58, 1482–1485.[CrossRef] [Google Scholar]
  34. Yoon, J. H., Kang, S. J., Oh, H. W. & Oh, T. K.(2007).Roseomonas terrae sp. nov. Int J Syst Evol Microbiol 57, 2485–2488.[CrossRef] [Google Scholar]
  35. Zhang, Y. Q., Yu, L. Y., Wang, D., Liu, H. Y., Sun, C. H., Jiang, W., Zhang, Y. Q. & Li, W. J.(2008).Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. Int J Syst Evol Microbiol 58, 2070–2074.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004812-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004812-0
Loading

Data & Media loading...

Supplements

Scanning electron micrograph of cells of strain CW67 grown in TSB for 2 days at 30 °C. Bar, 5 µm.

IMAGE

Fitch–Margoliash phylogenetic tree, based on almost-complete 16S rRNA gene sequences, showing the relationships between strain CW67 , members of the genus and related members of the family . [PDF](29 KB)

PDF

Fatty acid compositions of strain CW67 and type strains of species. [PDF](44 KB)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error