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Abstract

A Gram-negative, short ovoid- to coccus-shaped, aerobic, motile, non-spore-forming bacterium (designated strain KS-11) was isolated from sediment of the eutrophic Guanting reservoir in Beijing, China. Colonies grown on R2A agar plates were circular, convex and colourless to orange. The strain grew in the presence of up to 1 % NaCl (optimum, 0 % NaCl). Growth occurred at 25–40 °C (optimum, 28–37 °C) and at pH 6.0–9.5 (optimum, pH 7.5–9.0). On the basis of 16S rRNA gene sequence similarity, strain KS-11 was shown to belong to the class , being closely related to (96.9 % 16S rRNA gene sequence similarity), followed by (96.8 %), (96.8 %), (96.2 %) and (95.8 %). The major fatty acids of strain KS-11 were summed feature 7 (C 7/9/12) (83.8 %) and C (6.5 %) and the G+C content of the genomic DNA was 69.0 mol%. Based on comparative analysis of physiological and chemotaxonomic data, it is proposed that strain KS-11 represents a novel species of the genus , named sp. nov. The type strain is KS-11 (=CGMCC 1.7655=NBRC 104937).

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2009-11-01
2019-10-19
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References

  1. Chun, J., Lee, J. H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  2. Davis, D. H., Doudoroff, M., Stanier, R. Y. & Mandel, M. ( 1969; ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int J Syst Bacteriol 19, 375–390.[CrossRef]
    [Google Scholar]
  3. De Ley, J. ( 1970; ). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754.
    [Google Scholar]
  4. Dong, X. Z. & Cai, M. Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press.
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Gerhardt, P. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by N. R. Krieg. Washington, DC: American Society for Microbiology.
  7. Harker, M., Hirschberg, J. & Oren, A. ( 1998; ). Paracoccus marcusii sp. nov., an orange Gram-negative coccus. Int J Syst Bacteriol 48, 543–548.[CrossRef]
    [Google Scholar]
  8. Hildebrand, D. C., Palleroni, N. J., Hendson, M., Toth, J. & Johnson, J. L. ( 1994; ). Pseudomonas flavescens sp. nov., isolated from walnut blight cankers. Int J Syst Bacteriol 44, 410–415.[CrossRef]
    [Google Scholar]
  9. Khan, S. T., Takaichi, S. & Harayama, S. ( 2008; ). Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. Int J Syst Evol Microbiol 58, 383–386.[CrossRef]
    [Google Scholar]
  10. Kim, B. Y., Weon, H. Y. & Yoo, S. H. ( 2006; ). Paracoccus homiensis sp. nov., isolated from a sea-sand sample. Int J Syst Evol Microbiol 56, 2387–2390.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. La, H. J., Im, W. T., Ten, L. N., Kang, M. S., Shin, D. Y. & Lee, S. T. ( 2005; ). Paracoccus koreensis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludgeblanket (UASB) reactor. Int J Syst Evol Microbiol 55, 1657–1660.[CrossRef]
    [Google Scholar]
  13. Liu, Z. P., Wang, B. J., Liu, X. Y., Dai, X., Liu, Y. H. & Liu, S. J. ( 2008; ). Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. Int J Syst Evol Microbiol 58, 257–261.[CrossRef]
    [Google Scholar]
  14. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  15. Qu, J. H. & Yuan, H. L. ( 2008a; ). Sediminibacterium salmoneum gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 58, 2191–2194.[CrossRef]
    [Google Scholar]
  16. Qu, J. H., Li, H. F., Yang, J. S. & Yuan, H. L. ( 2008b; ). Flavobacterium cheniae sp. nov., isolated from sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 58, 2186–2190.[CrossRef]
    [Google Scholar]
  17. Rainey, F. A., Kelly, D. P., Stackbrandt, E., Burghardt, J., Hiraishi, A., Katayama, Y. & Wood, A. ( 1999; ). A re-evaluation of the taxonomy of Paracoccus denitrificans and a proposal for the combination Paracoccus pantotrophus comb. nov. Int J Syst Bacteriol 49, 645–651.[CrossRef]
    [Google Scholar]
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Siller, H., Fred, A. R., Stackebrandt, E. & Winter, J. ( 1996; ). Isolation and characterization of a new Gram-negative, acetone-degrading, nitrate-reducing bacterium from soil, Paracoccus solventivorans sp. nov. Int J Syst Bacteriol 46, 1125–1130.[CrossRef]
    [Google Scholar]
  21. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  22. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  24. Urakami, T., Araki, H., Oyanagi, H., Suzuki, K. & Komagata, K. ( 1990; ). Paracoccus aminophilus sp. nov. and Paracoccus aminovorans sp. nov., which utilize N,N-dimethylformamide. Int J Syst Bacteriol 40, 287–291.[CrossRef]
    [Google Scholar]
  25. Van Spanning, R. J. M., Stouthamer, A. H., Baker, S. C. & Van Verseveld, H. W. ( 2005; ). Genus XII. Paracoccus Davis 1969, 384AL emend. Ludwig, Mittenhuber and Friedrich 1993, 366. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria), pp. 197–204. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
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Minimum-evolution and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences. [PDF](28 KB)



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