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Volume 59, Issue 3

sp. nov., isolated from marine sediment of the East China Sea Free

Abstract

A Gram-negative, aerobic, neutrophilic, rod-shaped bacterium, designated strain CN85, was isolated from a sediment sample collected from the East China Sea and was subjected to a polyphasic taxonomic characterization. This isolate grew in the presence of 0.5–6.0 % (w/v) NaCl and at 20–40 °C; optimum growth was observed with 3 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed that Q-8 was the predominant respiratory quinone and that iso-C, iso-C 3-OH, iso-C 9, iso-C, iso-C and C were the major fatty acids. The G+C content of the genomic DNA was 57.8 mol%. On the basis of 16S rRNA gene sequence analysis, the isolate was affiliated to the genus . Strain CN85 exhibited most phylogenetic affinity with respect to the type strain of (97.0 % sequence similarity) and showed less than 97 % sequence similarity with respect to other described species with known 16S rRNA gene sequences. The DNA–DNA hybridization between strain CN85 and JCM 12187 was 44 %. On the basis of phenotypic and genotypic data, strain CN85 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CN85 (=CGMCC 1.7063 =JCM 15145).

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2009-03-01
2023-03-25
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Microbulbifer donghaiensis sp. nov., isolated from marine sediment of the East China Sea
Int J Syst Evol Microbiol 59, 545 (2009); https://doi.org/10.1099/ijs.0.004531-0
/content/journal/ijsem/10.1099/ijs.0.004531-0
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Supplements

vol. , part 3, pp. 545 - 549

Fatty acid compositions (%) of strain CN85 and the type strains of related species

Phenotypic characteristics that serve to differentiate strain CN85 from JCM 12187

[PDF file of Supplementary Tables S1 and S2](41 KB)

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