1887

Abstract

Anamorphic basidiomycetous yeast strains M9962 and M9963 were isolated from water samples collected from a small stream in Ohwakudani, Hakone, a hot-spring area in Japan. These belonged to a single species and were phylogenetically closely related to , although the ability to form ballistoconidia was not observed. Based on sequence analyses of the D1/D2 domain of the LSU rDNA and ITS regions and differences in G+C content, the name sp. nov. Takashima, Sugita, Toriumi et Nakase (Trichosporonales, Tremellomycetes, Basiomycota) is proposed for these isolates, with strain M9962 (=SP-5 =JCM 11965 =CBS 9427) as the type strain. The strains grew in YM broth at 47 °C and in YM broth with the pH adjusted to 1.5 by HCl, indicating that the species is thermotolerant and acid-tolerant.

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2009-01-01
2019-10-18
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References

  1. Barnett, J. A., Payne, R. W. & Yarrow, D. ( 2000; ). Yeasts: Characteristics and Identification, 3rd edn. Cambridge: Cambridge University Press.
  2. Deak, T. ( 2006; ). Environmental factors influencing yeasts. In Biodiversity and Ecophysiology of Yeasts (The Yeast Handbook series), pp. 156–174. Edited by C. A. Rosa & G. Péter. Berlin: Springer.
  3. Fell, J. W. & Statzell-Tallman, A. ( 1998; ). Cryptococcus Vuillemin. In The Yeasts, a Taxonomic Study, 4th edn, pp. 742–767. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  4. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A. ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef]
    [Google Scholar]
  5. Fell, J. W., Boekhout, T., Fonseca, A. & Sampaio, J. P. ( 2001; ). Basidiomycetous yeasts. In The Mycota, vol. 7, Systematics and Evolution, part B, pp. 3–35. Edited by D. J. McLaughlin, E. G. McLaughlin & P. A. Lemke. Berlin: Springer.
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Fungsin, B., Takashima, M., Sugita, T., Artjariyasripong, S., Potacharoen, W., Tanticharoen, M. & Nakase, T. ( 2006; ). Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov., two new ballistoconidium-forming yeast species in the Trichosporonales clade isolated from plant leaves in Thailand. J Gen Appl Microbiol 52, 73–81.[CrossRef]
    [Google Scholar]
  8. Itoh, T., Suzuki, K. & Nakase, T. ( 2002; ). Vulcanisaeta distributa gen. nov., sp. nov. and Vulcanisaeta souniana sp. nov., novel hyperthermophilic, rod-shaped crenarchaeotes isolated from hot springs in Japan. Int J Syst Evol Microbiol 52, 1097–1104.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Nakase, T. ( 1967; ). Microbiological studies on frozen foods. V. General properties of yeasts isolated from frozen foods. Food Hyg Soc Jpn 8, 53–57 (in Japanese).[CrossRef]
    [Google Scholar]
  11. Kurtzman, C. P. & Phaff, H. J. ( 1987; ). Molecular taxonomy. In The Yeasts, 2nd edn, vol. 1, pp. 63–94. Edited by A. H. Rose & J. S. Harrison. London: Academic Press.
  12. Kurtzman, C. P. & Robnett, C. J. ( 1997; ). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35, 1216–1223.
    [Google Scholar]
  13. Makimura, K., Murayama, Y. S. & Yamaguchi, H. ( 1994; ). Detection of a wide range of medically important fungal species by polymerase chain reaction (PCR). J Med Microbiol 40, 358–364.[CrossRef]
    [Google Scholar]
  14. Nagahama, T., Hamamoto, M., Nakase, T. & Horikoshi, K. ( 2003; ). Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically. Int J Syst Evol Microbiol 53, 897–903.[CrossRef]
    [Google Scholar]
  15. Nakase, T. & Suzuki, M. ( 1986; ). Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan. J Gen Appl Microbiol 32, 225–240.[CrossRef]
    [Google Scholar]
  16. Okoli, I., Oyeka, C. A., Kwon-Chung, K. J., Theelen, B., Robert, V., Groenewald, J. Z., McFadden, D. C., Casadevall, A. & Boekhout, T. ( 2007; ). Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar. FEMS Yeast Res 7, 339–350.[CrossRef]
    [Google Scholar]
  17. Péter, G., Tornai-Lehoczki, J., Shin, K. S. & Dlauchy, D. ( 2007; ). Ogataea thermophila sp. nov., the teleomorph of Candida thermophila. FEMS Yeast Res 7, 494–496.[CrossRef]
    [Google Scholar]
  18. Prillinger, H., Lopandic, K., Sugita, T. & Wuczkowski, M. ( 2007; ). Asterotremella gen. nov. albida, an anamorphic tremelloid yeast isolated from the agarics Asterophora lycoperdoides and Asterophora parastica. J Gen Appl Microbiol 53, 167–175.[CrossRef]
    [Google Scholar]
  19. Raspor, P. & Zupan, J. ( 2006; ). Yeast in extreme environment. In Biodiversity and Ecophysiology of Yeasts (The Yeast Handbook series), pp. 371–417. Edited by C. A. Rosa & G. Péter. Berlin: Springer.
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Scorzetti, G., Fell, J. W., Fonseca, A. & Statzell-Tallman, A. ( 2002; ). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2, 495–517.[CrossRef]
    [Google Scholar]
  22. Shin, K.-S., Shin, Y. K., Yoon, J.-H. & Park, Y.-H. ( 2001; ). Candida thermophila sp. nov., a novel thermophilic yeast isolated from soil. Int J Syst Evol Microbiol 51, 2167–2170.[CrossRef]
    [Google Scholar]
  23. Sugita, T. & Nakase, T. ( 1999; ). Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida. Syst Appl Microbiol 22, 79–86.[CrossRef]
    [Google Scholar]
  24. Sugita, T., Nishikawa, A., Ikeda, R. & Shinoda, T. ( 1999; ). Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification. J Clin Microbiol 37, 1985–1993.
    [Google Scholar]
  25. Takashima, M. & Nakase, T. ( 1999; ). Molecular phylogeny of the genus Cryptococcus and related species based on the sequences of 18S rDNA and internal transcribed spacer regions. Microbiol Cult Collect 15, 35–47.
    [Google Scholar]
  26. Takashima, M. & Nakase, T. ( 2000; ). Four new species of the genus Sporobolomyces isolated from leaves in Thailand. Mycoscience 41, 357–369.[CrossRef]
    [Google Scholar]
  27. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  29. Van Uden, N. ( 1984; ). Temperature profiles of yeasts. Adv Microb Physiol 25, 195–251.
    [Google Scholar]
  30. Walker, H. W. ( 1977; ). Spoilage of food by yeasts. Food Technol (Champaign) 31, 57–61.
    [Google Scholar]
  31. Watson, K. ( 1987; ). Temperature relations. In The Yeasts, 2nd edn, vol. 2, pp. 41–71. Edited by A. H. Rose & J. S. Harrison. London: Academic Press.
  32. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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