An ovoid to rod-shaped, phototrophic, purple non-sulfur bacterium was isolated from a brown-coloured microbial mat from the brackish water of Bhitarkanika mangrove forest, Dangmal, Orissa, India. Cells of strain JA296 were Gram-negative and motile, forming chains of four to eight cells. The colour of the cell suspension grown under anaerobic conditions in the light was yellowish green. Bacteriochlorophyll and the carotenoids spheroidene and spheroidenone of the spirilloxanthin series were present as photosynthetic pigments. The bacterium was a facultative anaerobe and was able to grow photo-organoheterotrophically and chemo-organoheterotrophically. Thiamine was required as a growth factor. C 7 was the dominant fatty acid. Internal cytoplasmic membranes were of the vesicular type. Strain JA296 did not require NaCl for growth. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA296 was most closely related to ATCC 11166 (95.5 % sequence similarity) and clustered with species of the genus of the family , class . On the basis of 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA296 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is JA296 (=JCM 14887 =CCUG 55130).


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Electron photomicrograph of an ultrathin section of cells of strain JA296 showing the vesicular nature of photosynthetic membranes extending throughout the cell. Bar, 333 nm.


Whole-cell absorption spectrum (a) and acetone spectrum of extracted pigments (b) of strain JA296 .

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