Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment

V. Venkata Ramana; P. Anil Kumar; T. N. R. Srinivas; Ch. Sasikala; Ch. V. Ramana

doi:10.1099/ijs.0.004507-0

Volume 59, Issue 5

Other

Free

Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment

V. Venkata Ramana¹, P. Anil Kumar², T. N. R. Srinivas², Ch. Sasikala² and Ch. V. Ramana¹
View Affiliations Hide Affiliations

Affiliations: ¹ 1Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad 500 046, India ² 2Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, JNT University, Kukatpally, Hyderabad 500 085, India
CorrespondenceCh. V. Ramana  [email protected]  or  [email protected]
Published: 01 May 2009 https://doi.org/10.1099/ijs.0.004507-0

Abstract

An ovoid to rod-shaped, phototrophic, purple non-sulfur bacterium was isolated from a brown-coloured microbial mat from the brackish water of Bhitarkanika mangrove forest, Dangmal, Orissa, India. Cells of strain JA296T were Gram-negative and motile, forming chains of four to eight cells. The colour of the cell suspension grown under anaerobic conditions in the light was yellowish green. Bacteriochlorophyll a and the carotenoids spheroidene and spheroidenone of the spirilloxanthin series were present as photosynthetic pigments. The bacterium was a facultative anaerobe and was able to grow photo-organoheterotrophically and chemo-organoheterotrophically. Thiamine was required as a growth factor. C18 : 1 ω7c was the dominant fatty acid. Internal cytoplasmic membranes were of the vesicular type. Strain JA296T did not require NaCl for growth. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA296T was most closely related to Rhodobacter capsulatus ATCC 11166T (95.5 % sequence similarity) and clustered with species of the genus Rhodobacter of the family Rhodobacteraceae, class Alphaproteobacteria. On the basis of 16S rRNA gene sequence analysis and morphological and physiological characteristics, strain JA296T represents a novel species of the genus Rhodobacter, for which the name Rhodobacter aestuarii sp. nov. is proposed; the type strain is JA296T (=JCM 14887T =CCUG 55130T).

Published Online: 01/05/2009

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004507-0

2009-05-01

2024-05-06

Full text loading...

/deliver/fulltext/ijsem/59/5/1133.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004507-0&mimeType=html&fmt=ahah

References

Anil Kumar, P., Srinivas, T. N. R., Sasikala, Ch. & Ramana, Ch. V.(2007).Rhodobacter changlensis sp. nov., a psychrotolerant, phototrophic alphaproteobacterium from the Himalayas of India. Int J Syst Evol Microbiol 57, 2568–2571.[CrossRef] [Google Scholar]
Arunasri, K., Venkata Ramana, V., Spröer, C., Sasikala, Ch. & Ramana, Ch. V.(2008).Rhodobacter megalophilus sp. nov., a phototroph from the Indian Himalayas possessing a wide temperature range for growth. Int J Syst Evol Microbiol 58, 1792–1796.[CrossRef] [Google Scholar]
Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O.(2005). PHYML Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33, W557–W559.[CrossRef] [Google Scholar]
Hansen, T. A. & Imhoff, J. F.(1985).Rhodobacter veldkampii, a new species of phototrophic purple nonsulfur bacteria. Int J Syst Bacteriol 35, 115–116.[CrossRef] [Google Scholar]
Hiraishi, A., Muramatsu, K. & Ueda, Y.(1996). Molecular genetic analyses of Rhodobacter azotoformans sp. nov. and related species of phototrophic bacteria. Syst Appl Microbiol 19, 168–177.[CrossRef] [Google Scholar]
Imhoff, J. F.(2005). Genus Rhodobacter Imhoff, Trüper and Pfennig 1984, 342^VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part C, pp. 161–167. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch. & Ramana, Ch. V.(2007a).Rhodovulum imhoffii sp. nov. Int J Syst Evol Microbiol 57, 228–232.[CrossRef] [Google Scholar]
Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Ramana, Ch. V. & Imhoff, J. F.(2007b).Rhodobacter vinaykumarii sp. nov., a marine phototrophic alphaproteobacterium from tidal waters, and emended description of the genus Rhodobacter. Int J Syst Evol Microbiol 57, 1984–1987.[CrossRef] [Google Scholar]
Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Spröer, C. & Ramana, Ch. V.(2008).Rhodobacter ovatus sp. nov., a phototrophic alphaproteobacterium isolated from a polluted pond. Int J Syst Evol Microbiol 58, 1379–1383.[CrossRef] [Google Scholar]
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
Venkata Ramana, V., Sasikala, Ch. & Ramana, Ch. V.(2008).Rhodobacter maris sp. nov., a phototrophic alphaproteobacterium isolated from a marine habitat of India. Int J Syst Evol Microbiol 58, 1719–1722.[CrossRef] [Google Scholar]

http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004507-0

Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment

Int J Syst Evol Microbiol 59, 1133 (2009); https://doi.org/10.1099/ijs.0.004507-0

/content/journal/ijsem/10.1099/ijs.0.004507-0

Data & Media loading...

Supplements

Supplementary Fig. S1. Electron photomicrograph of an ultrathin section of cells of strain JA296 Tshowing the vesicular nature of photosynthetic membranes extending throughout the cell. Bar, 333 nm.

Volume 59, Issue 5

Other

Free

Rhodobacter aestuarii sp. nov., a phototrophic alphaproteobacterium isolated from an estuarine environment

Abstract

Supplementary Fig. S1. Electron photomicrograph of an ultrathin section of cells of strain JA296 Tshowing the vesicular nature of photosynthetic membranes extending throughout the cell. Bar, 333 nm.

Supplementary Fig. S2. Whole-cell absorption spectrum (a) and acetone spectrum of extracted pigments (b) of strain JA296 T.

Most read this month

Most cited Most Cited RSS feed

Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies

A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae

DNA–DNA hybridization values and their relationship to whole-genome sequence similarities

OrthoANI: An improved algorithm and software for calculating average nucleotide identity

Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes

List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ

Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes

Valid publication of the names of forty-two phyla of prokaryotes

Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium

Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology