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Abstract

A Gram-negative, pale-yellow, non-spore-forming bacterium, motile with single polar flagella, strain E6, was isolated from coastal seawater collected from Tianjin, China, and its taxonomic position was investigated using a polyphasic approach. Strain E6 requires NaCl for growth and grows optimally at pH 8.2 and 36 °C and in the presence of 2.0 % (w/v) NaCl. It is positive for catalase and oxidase, and reduces nitrate to nitrite. The major fatty acids (>10 %) are C 7 (64.71 %) and 11-methyl C 7 (12.38 %), the ubiquinone system is Q-10 and the DNA G+C content is 53.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain E6 represented a new lineage in the and is related to genera , , and . Strain E6 shows highest 16S rRNA gene sequence similarity (93.3 %) to CL-GR60 and less than 92.2 % similarity to other relatives. It can be differentiated from its closest phylogenetic neighbours on the basis of several phenotypic features, including nitrate reduction, assimilation of -glucose, -arabinose, -mannose, mannitol and maltose and major fatty acid composition. A polyphasic analysis supported the conclusion that strain E6 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is E6 (=CGMCC 1.7002 =JCM 15144).

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2009-09-01
2020-01-26
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa com. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  3. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  5. Dong, X.-Z. & Cai, M.-Y. (editors) ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  6. Foster, G., Osterman, B. S., Godfroid, J., Jacques, I. & Cloeckaert, A. ( 2007; ). Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 57, 2688–2693.[CrossRef]
    [Google Scholar]
  7. Garrity, G. M., Bell, J. A. & Lilburn, T. ( 2005; ). Family VIII. Hyphomicrobiaceae Babudieri 1950, 589. In Bergey's Manual of Systematic Bacteriology, vol. 2, part C, pp. 476. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Hwang, C. Y. & Cho, B. C. ( 2008; ). Cucumibacter marinus gen. nov., sp. nov., a marine bacterium in the family Hyphomicrobiaceae. Int J Syst Evol Microbiol 58, 1591–1597.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lee, S.-D. ( 2007; ). Devosia subaequoris sp. nov., isolated from beach sediment. Int J Syst Evol Microbiol 57, 2212–2215.[CrossRef]
    [Google Scholar]
  13. Lee, K.-B., Liu, C.-T., Anzai, Y., Kim, H., Aono, T. & Oyaizu, H. ( 2005; ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteriaceae fam. nov. Int J Syst Evol Microbiol 55, 1907–1919.[CrossRef]
    [Google Scholar]
  14. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  15. Mergaert, J. & Swings, J. ( 2006; ). Family IV. Phyllobacteriaceae fam. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107. Int J Syst Evol Microbiol 56, 1–6.[CrossRef]
    [Google Scholar]
  16. Nakagawa, Y., Sakane, T. & Yokota, A. ( 1996; ). Transfer of ‘Pseudomonas riboflavina’ (Foster 1944), a Gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Syst Bacteriol 46, 16–22.[CrossRef]
    [Google Scholar]
  17. Rivas, R., Willems, A., Subba-Rao, N. S., Mateos, P. F., Dazzo, F. B., Kroppenstedt, R. M., Martínez-Molina, E., Gillis, M. & Velázquez, E. ( 2003; ). Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26, 47–53.[CrossRef]
    [Google Scholar]
  18. Rüger, H.-J. & Höfle, M. G. ( 1992; ). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov. nom. rev.; Agrobacterium gelatinovorum sp. nov. nom. rev.; and Agrobacterium stellulatum sp. nov. nom. rev. Int J Syst Bacteriol 42, 133–143.[CrossRef]
    [Google Scholar]
  19. Ryu, S.-H., Chung, B.-S., Le, N.-T., Jang, H.-H., Yun, P.-Y., Park, W. & Jeon, C.-O. ( 2008; ). Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea. Int J Syst Evol Microbiol 58, 633–636.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Teyssier, C., Marchandin, H., Jean-Pierre, H., Masnou, A., Dusart, G. & Jumas-Bilak, E. ( 2007; ). Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples. Int J Syst Evol Microbiol 57, 1007–1013.[CrossRef]
    [Google Scholar]
  22. Tripathi, A. K., Verma, S. C., Chowdhury, S. P., Lebuhn, M., Gattinger, A. & Schloter, M. ( 2006; ). Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India. Int J Syst Evol Microbiol 56, 1677–1680.[CrossRef]
    [Google Scholar]
  23. Trujillo, M. E., Willems, A., Abril, A., Planchuelo, A.-M., Rivas, R., Ludeña, D., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2005; ). Nodulation of Lupinus albus by strains of Ochrobactrum lupini sp. nov. Appl Environ Microbiol 71, 1318–1327.[CrossRef]
    [Google Scholar]
  24. Uchino, Y., Hirata, A., Yokota, A. & Sugiyama, J. ( 1998; ). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44, 201–210.[CrossRef]
    [Google Scholar]
  25. Vanparys, B., Heylen, K., Lebbe, L. & De Vos, P. ( 2005; ). Devosia limi sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55, 1997–2000.[CrossRef]
    [Google Scholar]
  26. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  27. Zhang, D., Yang, H., Zhang, W., Huang, Z. & Liu, S.-J. ( 2003; ). Rhodocista pekingensis sp. nov., a cyst-forming phototrophic bacterium from a municipal wastewater treatment plant. Int J Syst Evol Microbiol 53, 1111–1114.[CrossRef]
    [Google Scholar]
  28. Zurdo-Piñeiro, J. L., Rivas, R., Trujillo, M. E., Vizcaíno, N., Carrasco, J. A., Chamber, M., Palomares, A., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2007; ). Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain. Int J Syst Evol Microbiol 57, 784–788.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph of a cell of strain E6 grown on MA at 30 °C for 24 h. Bar, 1 µm.

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Comparison of fatty acid profiles of strain E6 and related strains. [PDF](67 KB)

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