Two novel spore-forming lactic acid bacteria, strains SL213 and SL1213, were isolated from vineyard soils in Korea. Cells of both isolates were rod-shaped bacilli and contained subterminal, ellipsoidal spores. Strains were facultatively anaerobic, catalase-positive, oxidase-negative and motile with single flagella. Diaminopimelic acid, glucose and galactose were detected in whole-cell hydrolysates. Major fatty acids found in the strains were anteiso-C, iso-C, iso-C, C and anteiso-C. The G+C contents of the DNA were 46.1 and 46.3 mol%. 16S rRNA gene sequences from the two strains were almost identical (99.9 %) and placed them in the genus , according to phylogenetic analysis. The type strains most closely related to SL213 were ATCC 7050 and ATCC 14574, with 16S rRNA gene sequence similarities of 96.9 and 95.9 %, respectively. Levels of DNA–DNA relatedness between strain SL213 and strain SL1213, ATCC 7050 and ATCC 14574 were 92.5, 49.0 and 27.5 %, respectively. On the basis of 16S rRNA gene sequences and chemotaxonomic and phenotypic evidence given in this study, we report that SL213 represents a novel species, for which the name sp. nov. is proposed. The type strain is SL213 (=KCTC 13078 =JCM 14638).


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