Three isolates, recovered from mussels (genus ), and one isolate from brackish water in Catalonia (north-east Spain) showed a novel pattern using a recently described identification method for members of the genus , 16S rRNA gene RFLP. Enterobacterial repetitive intergenic consensus PCR fingerprinting demonstrated that the three isolates from mussels belonged to two genotypes and that the fourth isolate from water belonged to a third genotype. Analysis of the 16S rRNA and gene sequences showed that the new isolates formed a separate lineage within the genus . This was also confirmed by the low DNA–DNA relatedness values (16–30 %) of the isolates with the type strains of recognized species. Hydrolysis of indoxyl acetate, a characteristic trait for all species of the genus , was negative for the novel isolates. The susceptibility of the novel isolates to cefoperazone, together with the lack of urease production and nitrate reduction, further enabled them to be differentiated from recognized species based on physiological characteristics. Genotypic and phenotypic characteristics indicated that the new isolates represent a novel species of the genus , for which the name sp. nov. is proposed, with the type strain F2075 (=CECT 7386 =LMG 24559). The DNA G+C content of strain F2075 was 26.9 mol%.


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Agarose gel showing ERIC-PCR patterns of sp. nov. [ PDF] 172 KB


Neighbour-joining phylogenetic tree showing the relationship of sp. nov. with other species of the genus on the basis of 487 nucleotides from the gene. [ PDF] 35 KB


Species and strains used in this study. [ PDF] 37 KB

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