1887

Abstract

Two novel anaerobic, moderately thermophilic and cellulose-/cellobiose-digesting bacteria, EBR45 and EBR596, were isolated from anaerobic sludge of a cellulose-degrading methanogenic bioreactor. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belonged to cluster III within the low-G+C-content Gram-positive bacteria. The close relatives of EBR45 were DSM 16021 (sequence identity, 94.6 %) and DSM 1237 (93.4 %). The closest relative of EBR596 was DSM 8532 (95.9 %). Both isolates were rod-shaped sporulators, growing optimally at 60 °C. EBR45 was Gram-staining-reaction-variable and non-motile, formed bright-yellow colonies on solid media, and grew on a relatively narrow range of carbohydrates including cellulose and cellobiose. EBR596 was Gram-staining-reaction-negative and motile, formed glossy white colonies and grew on cellobiose and various carbohydrates except cellulose. Major fatty acid compositions were 16 : 0 iso, 16 : 0 and 16 : 0 dimethylacetal (strain EBR45) and 15 : 0 iso, 16 : 0 iso, 15 : 0 anteiso and 17 : 0 anteiso (strain EBR596). The DNA G+C contents were 36.9 mol% (EBR45) and 51.1 mol% (EBR596). Based on the phenotypic and phylogenetic data and genomic distinctiveness, strains EBR45 and EBR596 represent two novel species, for which the names sp. nov. (type strain EBR45 =DSM 19732 =NBRC 101661) and sp. nov. (type strain EBR596 =DSM 19027 =NBRC 102590) are proposed.

Keyword(s): DMA, dimethylacetal
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2009-07-01
2020-01-20
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