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Abstract

A taxonomic study was carried out on a Gram-negative, rod-shaped, non-motile, halophilic bacterium, designated strain W11-2B, which was isolated from a pyrene-degrading consortium that was enriched from sediment from the Pacific Ocean. Growth was observed at salinities of 0.5–10 % and at temperatures of 10–41 °C. Strain W11-2B was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain W11-2B was related most closely to SS011B1-20 (95.8 % similarity) and HTCC2597 (95.7 %); levels of 16S rRNA gene sequence similarity between strain W11-2B and the type strains of other species tested were below 95.2 %. The dominant fatty acids of strain W11-2B were C 7 (32.1 % of the total), C cyclo (20.9 %), C 7 11-methyl (19.5 %), C (7.3 %), C (6.6 %) and C (3.8 %). The G+C content of the chromosomal DNA was 64.6 mol%. The above data were in good agreement with those of members of the genus . Based on morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain W11-2B is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W11-2B (=CCTCC AB 208224=LMG 24619=MCCC 1A01034).

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2009-05-01
2019-12-05
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
  2. Cho, J.-C. & Giovannoni, S. J. ( 2004; ). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54, 1129–1136.[CrossRef]
    [Google Scholar]
  3. Dong, X.-Z. & Cai, M.-Y. ( 2001; ). Determinative Manual for Routine Bacteriology. Beijing: Scientific Press (English translation).
  4. Gu, J., Guo, B., Wang, Y.-N., Yu, S.-L., Inamori, R., Qu, R., Ye, Y.-G. & Wu, X.-L. ( 2007; ). Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 57, 157–160.[CrossRef]
    [Google Scholar]
  5. Lin, K. Y., Sheu, S. Y., Chang, P. S., Cho, J. C. & Chen, W. M. ( 2007; ). Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. Int J Syst Evol Microbiol 57, 1625–1629.[CrossRef]
    [Google Scholar]
  6. Liu, C. L. & Shao, Z. Z. ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef]
    [Google Scholar]
  7. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  8. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  9. Rzhetsky, A. & Nei, M. ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095.
    [Google Scholar]
  10. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  11. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  12. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  13. Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H. ( 2003; ). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484.[CrossRef]
    [Google Scholar]
  14. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  15. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  16. Wang, B. J., Lai, Q. L., Cui, Z. S., Tan, T. F. & Shao, Z. Z. ( 2008; ). A pyrene-degrading consortium from deep-sea sediment of the west pacific and its key member Cycloclasticus sp. P1. Environ Microbiol 10, 1948–1963.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph of cells of strain W11-2B . [PDF](308 KB)

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Cellular fatty acid contents of strain W11-2B and related species. [PDF](48 KB)

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