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Abstract
A taxonomic study was carried out on a Gram-negative, rod-shaped, non-motile, halophilic bacterium, designated strain W11-2BT, which was isolated from a pyrene-degrading consortium that was enriched from sediment from the Pacific Ocean. Growth was observed at salinities of 0.5–10 % and at temperatures of 10–41 °C. Strain W11-2BT was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain W11-2BT was related most closely to Oceanicola nanhaiensis SS011B1-20T (95.8 % similarity) and Oceanicola batsensis HTCC2597T (95.7 %); levels of 16S rRNA gene sequence similarity between strain W11-2BT and the type strains of other species tested were below 95.2 %. The dominant fatty acids of strain W11-2BT were C18 : 1 ω7c (32.1 % of the total), C19 : 0 cyclo (20.9 %), C18 : 1 ω7c 11-methyl (19.5 %), C18 : 0 (7.3 %), C17 : 0 (6.6 %) and C16 : 0 (3.8 %). The G+C content of the chromosomal DNA was 64.6 mol%. The above data were in good agreement with those of members of the genus Oceanicola. Based on morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain W11-2BT is considered to represent a novel species of the genus Oceanicola, for which the name Oceanicola pacificus sp. nov. is proposed. The type strain is W11-2BT (=CCTCC AB 208224T=LMG 24619T=MCCC 1A01034T).
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