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A novel strain, designated Jip 10T, isolated from dried rice straw, was characterized by a polyphasic approach to clarify its taxonomic position. The isolate was Gram-negative, facultatively aerobic, heterotrophic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate clustered with the genus Mucilaginibacter in the phylum Bacteroidetes. 16S rRNA gene sequence similarities between strain Jip 10T and the type strains of Mucilaginibacter gracilis and Mucilaginibacter paludis were 93.7 and 93.6 %, respectively. The G+C content of the genomic DNA was 48.1 mol%. Chemotaxonomic data [major menaquinone MK-7 and major fatty acids iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c)] supported the affiliation of strain Jip 10T to the genus Mucilaginibacter. However, the results of physiological and biochemical tests allowed phenotypic differentiation of strain Jip 10T from other Mucilaginibacter species with validly published names. Therefore, strain Jip 10T (=KCTC 12639T =LMG 23488T) was classified in the genus Mucilaginibacter as the type strain of a novel species, for which the name Mucilaginibacter daejeonensis sp. nov. is proposed.