A novel Gram-positive-staining, catalase- and oxidase-positive, non-motile, non-spore-forming coccus, designated YIM-Y21, was isolated from a salt mine in Yunnan, south-west China. The strain was moderately halophilic, facultatively alkaliphilic and obligately aerobic. Colonies were white. Growth occurred with 1.0–30.0 % NaCl (optimum, 10.0 % NaCl), at pH 6.0–10.0 (optimum, pH 8.5) and at 5–40 °C (optimum, 25 °C). The major amino acid constituents of the cell wall were glycine and lysine. The major cellular fatty acids were anteiso-C, anteiso-C and iso-C. MK-6 was the predominant respiratory quinone, with MK-7 present in minor amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content was 46.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain YIM-Y21 was most closely related to the type strains of the nine recognized species of the genus . The sequence similarities between the isolate and the type strains of members of this genus were in the range of 90.6–93.8 %, which indicated that strain YIM-Y21 is a representative of a novel species within the genus . Together with the phenotypic characteristics and chemotaxonomic differences, these results support the proposal of a novel species of the genus , sp. nov., with YIM-Y21 (=CCTCC AA 207024 =DSM 19776 =KCTC 13192) as the type strain.


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vol. , part 4, pp. 874 - 879

Fatty acid compositions of strain YIM-Y21 and members of the genus . [PDF](48 KB)


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