1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 4

sp. nov., a halophilic bacterium from a salt mine Free

Abstract

A novel Gram-positive-staining, catalase- and oxidase-positive, non-motile, non-spore-forming coccus, designated YIM-Y21, was isolated from a salt mine in Yunnan, south-west China. The strain was moderately halophilic, facultatively alkaliphilic and obligately aerobic. Colonies were white. Growth occurred with 1.0–30.0 % NaCl (optimum, 10.0 % NaCl), at pH 6.0–10.0 (optimum, pH 8.5) and at 5–40 °C (optimum, 25 °C). The major amino acid constituents of the cell wall were glycine and lysine. The major cellular fatty acids were anteiso-C, anteiso-C and iso-C. MK-6 was the predominant respiratory quinone, with MK-7 present in minor amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content was 46.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain YIM-Y21 was most closely related to the type strains of the nine recognized species of the genus . The sequence similarities between the isolate and the type strains of members of this genus were in the range of 90.6–93.8 %, which indicated that strain YIM-Y21 is a representative of a novel species within the genus . Together with the phenotypic characteristics and chemotaxonomic differences, these results support the proposal of a novel species of the genus , sp. nov., with YIM-Y21 (=CCTCC AA 207024 =DSM 19776 =KCTC 13192) as the type strain.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003251-0
2009-04-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/874.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003251-0&mimeType=html&fmt=ahah

References

  1. Amoozegar, M. A., Schumann, P., Hajighasemi, M., Ashengroph, M. & Razavi, M. R. ( 2008; ). Salinicoccus iranensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 58, 178–183.[CrossRef]
    [Google Scholar]
  2. Aslam, Z., Lim, J. H., Im, W.-T., Yasir, M., Chung, Y. R. & Lee, S.-T. ( 2007; ). Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57, 633–638.[CrossRef]
    [Google Scholar]
  3. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L. ( 2007a; ). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef]
    [Google Scholar]
  4. Chen, Y.-G., Li, H.-M., Li, Q.-Y., Chen, W. & Cui, X.-L. ( 2007b; ). Phylogenetic diversity of culturable bacteria in the ancient salt deposits of the Yipinglang Salt Mine, P. R. China. Wei Sheng Wu Xue Bao 47, 571–577 (in Chinese).
     [Google Scholar]
  5. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  6. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
     [Google Scholar]
  8. Doetsch, R. N. ( 1981; ). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  9. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Franca, L., Rainey, F. A., Nobre, M. F. & Costa, M. S. ( 2006; ). Salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate. Extremophiles 10, 531–536.[CrossRef]
    [Google Scholar]
  13. Gregersen, T. ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef]
    [Google Scholar]
  14. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  15. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M. ( 1985; ). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces – a Laboratory Manual, pp. 79–80. Edited by D. A. Hopwood, M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward & H. Schrempf. Norwich: John Innes Foundation.
  16. Hoyles, L., Collins, M. D., Foster, G., Falsen, E. & Schumann, P. ( 2004; ). Jeotgalicoccus pinnipedialis sp. nov., from a southern elephant seal (Mirounga leonina). Int J Syst Evol Microbiol 54, 745–748.[CrossRef]
    [Google Scholar]
  17. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  18. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  19. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  20. Lim, J.-M., Jeon, C. O., Song, S. M. & Kim, C.-J. ( 2005; ). Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 165–170.[CrossRef]
    [Google Scholar]
  21. Marquez, M. C., Ventosa, A. F. & Berraquero, R. ( 1990; ). Marinococcus hispanicus, a new species of moderately halophilic Gram-positive cocci. Int J Syst Bacteriol 40, 165–169.[CrossRef]
    [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  23. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  24. Pakdeeto, A., Tanasupawat, S., Thawai, C., Moonmangmee, S., Kudo, T. & Itoh, T. ( 2007; ). Salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57, 2004–2008.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
     [Google Scholar]
  26. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  27. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
     [Google Scholar]
  28. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  29. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  30. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
     [Google Scholar]
  31. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  32. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
     [Google Scholar]
  33. Ventosa, A., Marquez, M. C., Ruiz-Berraquero, F. & Kocur, M. ( 1990; ). Salinicoccus roseus gen. nov., sp. nov., a new moderately halophilic Gram-positive coccus. Syst Appl Microbiol 13, 29–33.[CrossRef]
    [Google Scholar]
  34. Ventosa, A., Marquez, M. C., Weiss, N. & Tindall, B. J. ( 1992; ). Transfer of Marinococcus hispanicus to the genus Salinicoccus as Salinicoccus hispanicus comb. nov. Syst Appl Microbiol 15, 530–534.[CrossRef]
    [Google Scholar]
  35. Yoon, J.-H., Lee, K.-C., Weiss, N., Kang, K. H. & Park, Y.-H. ( 2003; ). Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal. Int J Syst Evol Microbiol 53, 595–602.[CrossRef]
    [Google Scholar]
  36. Zhang, W., Xue, Y., Ma, Y., Zhou, P., Ventosa, A. & Grant, W. D. ( 2002; ). Salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 52, 789–793.[CrossRef]
    [Google Scholar]
  37. Zhang, Y.-Q., Yu, L.-Y., Liu, H.-Y., Zhang, Y.-Q., Xu, L.-H. & Li, W.-J. ( 2007; ). Salinicoccus luteus sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 57, 1901–1905.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003251-0
Loading
Salinicoccus albus sp. nov., a halophilic bacterium from a salt mine
Int J Syst Evol Microbiol 59, 874 (2009); https://doi.org/10.1099/ijs.0.003251-0
/content/journal/ijsem/10.1099/ijs.0.003251-0
/content/journal/ijsem/10.1099/ijs.0.003251-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 874 - 879

Fatty acid compositions of strain YIM-Y21 and members of the genus . [PDF](48 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error