1887

Abstract

Three actinomycetes, designated strains VN05A0342, VN05A0351 and VN05A0415, were isolated from plant-litter samples collected in the north of Vietnam and examined in a polyphasic taxonomic study. Phylogenetic analysis based on the 16S rRNA gene sequences showed that these isolates were most closely related to the type strain of (98.5 % sequence similarity). Morphological properties (the formation of spore domes and motile spores) and chemotaxonomic data supported the assignment of the three isolates to the genus . The isolates all contained the following: -diaminopimelic acid in the peptidoglycan (with small amounts of the isomer); ribose, mannose, galactose and glucose as the whole-cell sugars; MK-9(H) as the predominant isoprenoid quinone; C and C as the major cellular fatty acids; and phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol as the phospholipids. The high DNA–DNA relatedness (>71 %) among the three isolates showed that they represented a single species. On the other hand, the DNA–DNA relatedness between the novel isolates and all type strains of species was less than 46 %. The physiological properties of our isolates were distinct from those of all of the species with validly published names, e.g. decomposition of -tyrosine and aesculin and the utilization of raffinose and -arabitol. Therefore, strains VN05A0342, VN05A0351 and VN05A0415 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is VN05A0415 (=VTCC-A-0961 =NBRC 104154).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002907-0
2009-03-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/3/550.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002907-0&mimeType=html&fmt=ahah

References

  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Goodfellow, M. ( 1971; ). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69, 33–80.[CrossRef]
    [Google Scholar]
  4. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  6. Hayakawa, M. & Nonomura, H. ( 1987; ). Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef]
    [Google Scholar]
  7. Hayakawa, M., Otoguro, M., Takeuchi, T., Yamazaki, T. & Iinuma, Y. ( 2000; ). Application of a method incorporating differential centrifugation for selective isolation of motile actinomycetes in soil and plant litter. Antonie Van Leeuwenhoek 78, 171–185.[CrossRef]
    [Google Scholar]
  8. Itoh, T., Kudo, T., Parenti, F. & Seino, A. ( 1989; ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39, 168–173.[CrossRef]
    [Google Scholar]
  9. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  10. Kudo, T., Matsushima, K., Itoh, T., Sasaki, J. & Suzuki, K. ( 1998; ). Description of four new species of the genus Kineosporia: Kineosporia succinea sp. nov., Kineosporia rhizophila sp. nov., Kineosporia mikuniensis sp. nov. and Kineosporia rhamnosa sp. nov., isolated from plant samples, and amended description of the genus Kineosporia. Int J Syst Bacteriol 48, 1245–1255.[CrossRef]
    [Google Scholar]
  11. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  14. Nozawa, Y., Sakai, N., Arai, K., Kawasaki, Y. & Harada, K. ( 2007; ). Reliable and sensitive analysis of amino acids in the peptidoglycan of actinomycetes using the advanced Marfey's method. J Microbiol Methods 70, 306–311.[CrossRef]
    [Google Scholar]
  15. Pagani, H. & Parenti, F. ( 1978; ). Kineosporia, a new genus of the order Actinomycetales. Int J Syst Bacteriol 28, 401–406.[CrossRef]
    [Google Scholar]
  16. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  19. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  20. Tamura, T. & Hatano, K. ( 2001; ). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef]
    [Google Scholar]
  21. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Willoughby, L. G. ( 1969; ). A study on aquatic actinomycetes, the allochthonous leaf component. Nova Hedwigia 18, 45–113.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002907-0
Loading
/content/journal/ijsem/10.1099/ijs.0.002907-0
Loading

Data & Media loading...

vol. , part 3, pp. 550 - 554

Cultural characteristics of strains VN05A0342, VN05A0351 and VN05A0415 and the type strains of all species

Cellular fatty acid compositions of strains VN05A0342, VN05A0351 and VN05A0415 and the type strains of all species

[PDF file of Supplementary Tables S1 and S2](45 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error