1887

Abstract

Eight anaerobic, pigmented, non-spore-forming, Gram-negative, rod-shaped strains isolated from monkey oral cavities were characterized phenotypically and chemotaxonomically and their phylogenetic positions were determined using 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that these isolates represent a single species of the genus . These strains were most closely related to ATCC 25611, with 95.0 % 16S rRNA gene sequence similarity. The next most closely related species were and (92.7 and 92.1 % similarity to the respective type strains). The phenotypic and biochemical characteristics of the isolates were the same as those of JCM 12248 and JCM 12250. The isolates could be differentiated from JCM 11140 on the basis of mannose fermentation and -fucosidase activity. The isolates could not be distinguished from or using conventional biochemical tests. DNA–DNA hybridization experiments revealed the genomic distinctiveness of these eight strains with respect to JCM 11140, JCM 12248 and JCM 12250. On the basis of these data, strains 04013, 04021, 04043, 04052, 0406, 04113, 04111 and 04161 represent a novel species, for which the name sp. nov. is proposed. The type strain is 04052 (=JCM 15124 =CCUG 56137).

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2009-02-01
2020-01-22
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vol. , part 2, pp. 319 - 322

Sequence of the primer pair for and alignment of the 16S rRNA gene sequences of related species. [PDF](464 KB)



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