1887

Abstract

A novel aerobic, psychrotolerant, yellow-to-orange bacterium (strain 0423) was isolated from the China No. 1 glacier. Strain 0423 displayed phenotypic and chemotaxonomic features of the genus , containing anteiso-C and iso-C as the major fatty acids. The temperature range for growth was 4–28 °C, with optimum growth at 20–21 °C. The genomic DNA G+C content was 49 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0423 was related to members of the genus , sharing the highest sequence similarities with the type strains of , , and . On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species, sp. nov., is proposed. The type strain is 0423 (=CGMCC 1.6846 =JCM 15088).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002592-0
2009-06-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1387.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002592-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  2. Dai, X., Wang, Y.-N., Wang, B.-J., Liu, S.-J. & Zhou, Y.-G.(2005).Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov. Int J Syst Evol Microbiol 55, 699–702.[CrossRef] [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  4. Dong, X.-Z. & Cai, M.-Y. (editors)(2001). Determination of biochemical properties. In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing: Science Press (in Chinese).
  5. Engelhardt, M. A., Daly, K., Swannell, R. P. J. & Head, I. M.(2001). Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal sediment, and description of Planococcus alkanoclasticus sp. nov. J Appl Microbiol 90, 237–247.[CrossRef] [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  7. Junge, K., Gosink, J. J., Hoppe, H.-G. & Staley, J. T.(1998).Arthrobacter, Brachybacterium and Planococcus isolates identified from Antarctic sea ice brine. Description of Planococcus mcmeekinii, sp. nov. Syst Appl Microbiol 21, 306–314.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  12. Nakagawa, Y., Sakane, T. & Yokota, A.(1996). Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46, 866–870.[CrossRef] [Google Scholar]
  13. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S.(2002).Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T. & Sangkhobol, V.(1986). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef] [Google Scholar]
  18. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J.(1989). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178. [Google Scholar]
  19. Yoon, J.-H., Kang, S.-S., Lee, K.-C., Lee, E. S., Kho, Y. H., Kang, K. E. & Park, Y.-H.(2001).Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al. 1996) and Planococcus mcmeekinii (Junge et al. 1998) to the genus Planomicrobium. Int J Syst Evol Microbiol 51, 1511–1520. [Google Scholar]
  20. Zhang, D.-C., Wang, H.-X., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J.(2006).Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56, 2921–2925.[CrossRef] [Google Scholar]
  21. Zhang, D.-C., Wang, H.-X., Cui, H.-L., Yang, Y., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J.(2007).Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier. Int J Syst Evol Microbiol 57, 866–869.[CrossRef] [Google Scholar]
  22. Zhang, D.-C., Liu, H.-C., Xin, Y.-H., Yu, Y., Zhou, P.-J. & Zhou, Y.-G.(2008).Salinibacterium xinjiangense sp. nov., a psychrophilic bacterium isolated from the China No. 1 glacier. Int J Syst Evol Microbiol 58, 2739–2742.[CrossRef] [Google Scholar]
  23. Zhu, F., Wang, S. & Zhou, P.(2003).Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53, 853–857.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002592-0
Loading
/content/journal/ijsem/10.1099/ijs.0.002592-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1387 - 1390

Cellular fatty acid profiles of sp. nov. 0423 and its phylogenetic relatives. [PDF](48 KB)



PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error