Pu'er tea is a fermented drink made from the leaves of the tea plant, . Two novel bacteria, designated strains b09i-3 and b13i-1, were isolated during the process of fermentation of this tea. These isolates were Gram-positive, endospore-forming, motile rods that grew at 25–42 °C and pH 5.5–10.4. The DNA G+C content was 56.6–58.4 mol%, the predominant isoprenoid quinone was MK-7 and the predominant cellular fatty acid was anteiso-C (49.0–50 % of the total). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains b09i-3 and b13i-1 shared 99.9 % similarity and were affiliated with a cluster within the family . Strains b09i-3 and b13i-1 were related most closely to DCY16 (97 % 16S rRNA gene sequence similarity). Levels of DNA–DNA relatedness between the two novel isolates and DCY16 were below 56 %. The phylogenetic and phenotypic characteristics of these novel isolates allowed them to be distinguished clearly from recognized species of the genus . Based on these data, strains b09i-3 and b13i-1 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type species is b09i-3 (=KCTC 13223=CECT 7360).


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vol. , part 5, pp. 1002 - 1006

Transmission electron micrograph of cells of strain b09i-3 . [PDF](263 KB)


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