1887

Abstract

Pu'er tea is a fermented drink made from the leaves of the tea plant, . Two novel bacteria, designated strains b09i-3 and b13i-1, were isolated during the process of fermentation of this tea. These isolates were Gram-positive, endospore-forming, motile rods that grew at 25–42 °C and pH 5.5–10.4. The DNA G+C content was 56.6–58.4 mol%, the predominant isoprenoid quinone was MK-7 and the predominant cellular fatty acid was anteiso-C (49.0–50 % of the total). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains b09i-3 and b13i-1 shared 99.9 % similarity and were affiliated with a cluster within the family . Strains b09i-3 and b13i-1 were related most closely to DCY16 (97 % 16S rRNA gene sequence similarity). Levels of DNA–DNA relatedness between the two novel isolates and DCY16 were below 56 %. The phylogenetic and phenotypic characteristics of these novel isolates allowed them to be distinguished clearly from recognized species of the genus . Based on these data, strains b09i-3 and b13i-1 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type species is b09i-3 (=KCTC 13223=CECT 7360).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002352-0
2009-05-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/1002.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002352-0&mimeType=html&fmt=ahah

References

  1. Ash, C., Priest, F. G. & Collins, M. D. ( 1993; ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64, 253–260.
    [Google Scholar]
  2. Chen, C. N., Lin, C. P., Huang, K. K., Chen, W. C., Hsieh, H. P., Liang, P. H. & Hsu, J. T. ( 2005; ). Inhibition of SARS-CoV 3C-like protease activity by theaflavin-3,3′-digallate (TF3). Evid Based Complement Alternat Med 2, 209–215.[CrossRef]
    [Google Scholar]
  3. Claus, D. & Berkeley, R. C. W. ( 1986; ). Genus Bacillus Cohn 1872, 174AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1139. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  4. Collins, M. D. & Jones, D. ( 1981; ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45, 316–354.
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Heyndrickx, M., Vandemeulebroecke, K., Scheldeman, P., Hoste, B., Kersters, K., De Vos, P., Logan, N. A., Aziz, A. M., Ali, N. & Berkeley, R. C. ( 1995; ). Paenibacillus (formerly Bacillus) gordonae (Pichinoty et al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus. Int J Syst Bacteriol 45, 661–669.[CrossRef]
    [Google Scholar]
  7. Kim, M. K., Kim, Y.-A., Park, M.-J. & Yang, D.-C. ( 2008; ). Paenibacillus ginsengihumi sp. nov., a bacterium isolated from soil in a ginseng field. Int J Syst Evol Microbiol 58, 1164–1168.[CrossRef]
    [Google Scholar]
  8. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  9. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  10. Kusuda, R., Kawai, K., Salati, F., Banner, C. R. & Fryer, J. L. ( 1991; ). Enterococcus seriolicida sp. nov., a fish pathogen. Int J Syst Bacteriol 41, 406–409.[CrossRef]
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  12. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Lee, J.-S., Lee, K. C., Pyun, Y.-R. & Bae, K. S. ( 2003; ). Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. Int J Syst Evol Microbiol 53, 1277–1280.[CrossRef]
    [Google Scholar]
  14. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T., Jr, Saxman, P. R., Farris, R. J., Garrity, G. M., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M. ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  15. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  16. Paster, B. J. & Canale-Parola, E. ( 1982; ). Physiological diversity of rumen spirochetes. Appl Environ Microbiol 43, 686–693.
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sambrook, J. & Russell, D. W. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  20. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  21. Skipper, N., Sutherland, M., Davies, R. W., Kilburn, D., Miller, R. C., Jr, Warren, A. & Wong, R. ( 1985; ). Secretion of a bacterial cellulase by yeast. Science 230, 958–960.[CrossRef]
    [Google Scholar]
  22. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  23. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  24. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  27. Willcox, M. D. P. ( 1996; ). Identification and classification of species within the Streptococcus sanguis group. Aust Dent J 41, 107–112.[CrossRef]
    [Google Scholar]
  28. Woese, C. R. ( 1987; ). Bacterial evolution. Microbiol Rev 51, 221–271.
    [Google Scholar]
  29. Yoon, J. H., Seo, W. T., Shin, Y. K., Kho, Y. H., Kang, K. H. & Park, Y. H. ( 2002; ). Paenibacillus chinjuensis sp. nov., a novel exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 52, 415–421.
    [Google Scholar]
  30. Yoon, J.-H., Kim, H., Kim, I.-G., Kang, K. H. & Park, Y.-H. ( 2003; ). Erythrobacter flavus sp. nov., a slight halophile from the East Sea in Korea. Int J Syst Evol Microbiol 53, 1169–1174.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002352-0
Loading
/content/journal/ijsem/10.1099/ijs.0.002352-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1002 - 1006

Transmission electron micrograph of cells of strain b09i-3 . [PDF](263 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error