A novel heterotrophic, marine, strictly aerobic, motile bacterium was isolated from the Red Sea at a depth of 1 m. Analysis of its 16S rRNA gene sequence, retrieved from the whole-genome sequence, showed that this bacterium was most closely related to the genera , and , each of which contains a single species, within the class . Phenotypic, genotypic and phylogenetic analyses supported the creation of a novel genus and species to accommodate this bacterium, for which the name gen. nov., sp. nov. is proposed. The type strain of is RED65 (=CECT 7074 =CCUG 52064).


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  1. Arahal, D. R., Lekunberri, I., González, J. M., Pascual, J., Pujalte, M. J., Pedrós-Alió, C. & Pinhassi, J.(2007).Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium. Int J Syst Evol Microbiol 57, 1000–1006.[CrossRef] [Google Scholar]
  2. Baumann, P. & Baumann, L.(1981). The marine gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes. In The Prokaryotes, vol. 2, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows, H. Schleger. Berlin: Springer.
  3. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D.(1972). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429. [Google Scholar]
  4. Bowditch, R. D., Baumann, L. & Baumann, P.(1984). Description of Oceanospirillum kriegii sp. nov. and O. jannaschii sp. nov. and assignment of two species of Alteromonas to this genus as O. commune comb. nov. and O. vagum comb. nov. Curr Microbiol 10, 221–230.[CrossRef] [Google Scholar]
  5. Giovannoni, S. J. & Rappé, M.(2000). Evolution, diversity, and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans, pp. 47–84. Edited by D. Kirchman. New York: Wiley.
  6. Hagström, Å., Pommier, T., Rohwer, F., Simum, K., Stolte, W., Svensson, D. & Zweifel, U. L.(2002). Use of 16S ribosomal DNA for delineation of marine bacterioplankton species. Appl Environ Microbiol 68, 3628–3633.[CrossRef] [Google Scholar]
  7. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  8. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H.(1998). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef] [Google Scholar]
  9. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  10. Macián, M. C., Ludwig, W., Schleifer, K.-H., Garay, E. & Pujalte, M. J.(2001).Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. Int J Syst Evol Microbiol 51, 1283–1289. [Google Scholar]
  11. Macián, M. C., Arahal, D. R., Garay, E., Ludwig, W., Schleifer, K.-H. & Pujalte, M. J.(2005).Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55, 105–110.[CrossRef] [Google Scholar]
  12. Pinhassi, J. & Berman, T.(2003). Differential growth response of colony-forming α- and γ-proteobacteria in dilution culture and nutrient addition experiments from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat. Appl Environ Microbiol 69, 199–211.[CrossRef] [Google Scholar]
  13. Pinhassi, J., Bowman, J. P., Nedashkovskaya, O. I., Lekunberri, I., Gómez-Consarnau, L. & Pedrós-Alió, C.(2006).Leeuwenhoekiella blandensis sp. nov., a genome-sequenced marine member of the family Flavobacteriaceae. Int J Syst Evol Microbiol 56, 1489–1493.[CrossRef] [Google Scholar]
  14. Pinhassi, J., Pujalte, M. J., Macián, M. C., Lekunberri, I., González, J. M., Pedrós-Alió, C. & Arahal, D. R.(2007).Reinekea blandensis sp. nov., a marine, genome-sequenced gammaproteobacterium. Int J Syst Evol Microbiol 57, 2370–2375.[CrossRef] [Google Scholar]
  15. Sakane, T. & Yokota, A.(1994). Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum, Magnetospirillum and Oceanospirillum. Syst Appl Microbiol 17, 128–134.[CrossRef] [Google Scholar]
  16. Trüper, H. G. & Schleifer, K. H.(2006). Prokaryote characterization and identification. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 1, pp. 58–79. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  17. Yakimov, M. M., Giuliano, L., Gentile, G., Crisafi, E., Chernikova, T. N., Abraham, W.-R., Lünsdorf, H., Timmis, K. N. & Golyshin, P. N.(2003).Oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water. Int J Syst Evol Microbiol 53, 779–785.[CrossRef] [Google Scholar]
  18. Yakimov, M. M., Giuliano, L., Denaro, R., Crisafi, E., Chernikova, T. N., Abraham, W.-R., Luensdorf, H., Timmis, K. N. & Golyshin, P. N.(2004).Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. Int J Syst Evol Microbiol 54, 141–148.[CrossRef] [Google Scholar]
  19. ZoBell, C. E.(1946). In Marine Microbiology, vol. XVII, p. 240. Edited by F. Verdoorn. Waltham, MA: Chronica Botanica.

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Maximum-parsimony (Fig. S1) and maximum-likelihood (Fig. S2) phylogenetic trees, based on almost-complete 16S rRNA gene sequences of gen. nov., sp. nov. RED65 and closely related species. [PDF](23 KB)


Scanning electron micrograph of cells of RED65 at stationary growth phase, immobilized on a 0.2-µm-pore-size polycarbonate filter. Bar, 5 µm.

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