1887

Abstract

Three strains of an unidentified Gram-stain-variable, fastidious, catalase-negative, capnophilic, non-spore-forming, coccus-shaped bacterium from human wound specimens were characterized by phenotypic and molecular taxonomic methods. Initially, these strains were anaerobic; with repeated culture, they became aerotolerant. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains were genealogically homogeneous and constituted a novel subline within the genus . The unknown bacterium was readily distinguished from other species by biochemical tests. On the basis of both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from clinical specimens be classified as sp. nov. The type strain is WAL 1945J (=ATCC BAA-1630 =CCUG 57045).

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2010-05-01
2019-10-23
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References

  1. Benson, D. A., Boguski, M. S., Lipman, D. J. & Ostell, J. ( 1997; ). GenBank. Nucleic Acids Res 25, 1–6.[CrossRef]
    [Google Scholar]
  2. Berger, U. ( 1992; ) The genus Gemella. In The Prokaryotes, 2nd edn, pp. 1643–1653. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  3. Collins, M. D., Hutson, R. A., Falsen, E., Sjöden, B. & Facklam, R. R. ( 1998a; ). Gemella bergeriae sp. nov., isolated from human clinical specimens. J Clin Microbiol 36, 1290–1293.
    [Google Scholar]
  4. Collins, M. D., Hutson, R. A., Falsen, E., Sjöden, B. & Facklam, R. R. ( 1998b; ). Description of Gemella sanguinis sp. nov., isolated from human clinical specimens. J Clin Microbiol 36, 3090–3093.
    [Google Scholar]
  5. Collins, M. D., Rodriguez Jovita, M., Foster, G., Sjödén, B. & Falsen, E. ( 1999; ). Characterization of a Gemella-like organism from the oral cavity of a dog: description of Gemella palaticanis sp. nov. Int J Syst Bacteriol 49, 1523–1526.[CrossRef]
    [Google Scholar]
  6. Facklam, R. & Elliott, J. A. ( 1995; ). Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8, 479–495.
    [Google Scholar]
  7. Hoyles, L., Foster, G., Falsen, E. & Collins, M. D. ( 2000; ). Characterization of a Gemella-like organism isolated from an abscess of a rabbit: description of Gemella cuniculi sp. nov. Int J Syst Evol Microbiol 50, 2037–2041.[CrossRef]
    [Google Scholar]
  8. Jousimies-Somer, H., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M. ( 2002; ). Wadsworth–KTL Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing.
  9. Song, Y., Liu, C., McTeague, M. & Finegold, S. M. ( 2003; ). 16S ribosomal DNA sequence-based analysis of clinically significant gram-positive anaerobic cocci. J Clin Microbiol 41, 1363–1369.[CrossRef]
    [Google Scholar]
  10. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity for progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
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