1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 4

sp. nov., from fermented fish () in Thailand Free

Abstract

A Gram-negative, facultatively anaerobic, moderately halophilic bacterium, strain ND1-1, was isolated from fermented fish () in Thailand. The cells were curved rods, motile and non-endospore-forming. The novel strain grew optimally at 37 °C, at pH 8 and in the presence of 9−10 % (w/v) NaCl. The predominant respiratory lipoquinone was Q-8. The major cellular fatty acids were C and C. Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 49.0 mol%. Comparative 16S rRNA gene sequence analyses indicated that strain ND1-1 was closely related to , which comprises three subspecies, and with gene sequence similarities of 98.3–98.6 %. Strain ND1-1 showed low levels of DNA–DNA relatedness with subsp. JCM 15095 (33.2 %), subsp. DSM 16359 (38.4 %), subsp. JCM 15096 (59.7 %), and AF-2004 (42.1 %). On the basis of the physiological and biochemical characteristics and the molecular data presented, strain ND1-1 should be classified as a novel species of the genus for which the name sp. nov. is proposed. The type strain is ND1-1 (=JCM 14472=PCU 301=TISTR 1810).

  • Published Online:
Keyword(s): DPG, diphosphatidylglycerol , PE, phosphatidylethanolamine and PG, phosphatidylglycerol
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.001768-0
2009-04-01
2020-04-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/880.html?itemId=/content/journal/ijsem/10.1099/ijs.0.001768-0&mimeType=html&fmt=ahah

References

  1. Amoozegar, M. A., Schumann, P., Hajighasemi, M., Fatemi, Z. A. & Karbalaei-Heidari, R. H. ( 2008; ). Salinivibrio proteolyticus sp. nov., a moderately halophilic and proteolytic species from a hypersaline lake in Iran. Int J Syst Evol Microbiol 58, 1159–1163.[CrossRef]
    [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn, 331 pp. Cambridge: Cambridge University Press.
  3. Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. ( 1966; ). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45, 493–496.
     [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Forbes, L. ( 1981; ). Rapid flagella stain. J Clin Microbiol 13, 807–809.
     [Google Scholar]
  7. Garcia, M. T., Ventosa, A., Ruiz-Berraquero, F. & Kocur, M. ( 1987; ). Taxonomic study and amended description of Vibrio costicola. Int J Syst Bacteriol 37, 251–256.[CrossRef]
    [Google Scholar]
  8. Huang, C. Y., Garcia, J. L., Patel, B. K. C., Cayol, J. L., Baresi, L. & Mah, R. A. ( 2000; ). Salinivibrio costicola subsp. vallismortis subsp. nov., a halotolerant facultative anaerobe from Death Valley, and emended description of Salinivibrio costicola. Int J Syst Evol Microbiol 50, 615–622.[CrossRef]
    [Google Scholar]
  9. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
     [Google Scholar]
  10. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
     [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
     [Google Scholar]
  13. Mellado, E., Moore, E. R. B., Nieto, J. J. & Ventosa, A. ( 1996; ). Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol 46, 817–821.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  15. Namwong, S., Tanasupawat, S., Smitinont, T., Visessanguan, W., Kudo, T. & Itoh, T. ( 2005; ). Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 55, 315–320.[CrossRef]
    [Google Scholar]
  16. Pakdeeto, A., Tanasupawat, S., Thawai, C., Moonmangmee, S., Kudo, T. & Itoh, T. ( 2007; ). Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 57, 364–369.[CrossRef]
    [Google Scholar]
  17. Perrière, G. & Gouy, M. ( 1996; ). WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78, 364–369.[CrossRef]
    [Google Scholar]
  18. Phithakpol, B., Varanyanond, W., Reungmaneepaitoon, S. & Wood, H. ( 1995; ). The Traditional Fermented Foods of Thailand. Kuala Lumpur: ASEAN Food Handling Bureau Level 3.
  19. Romanenko, L. A., Uchino, M., Mikhailov, V. V., Zhukova, V. N. & Uchimura, T. ( 2003; ). Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53, 829–832.[CrossRef]
    [Google Scholar]
  20. Romano, I., Gambacorta, A., Lama, L., Nicolaus, B. & Giordano, A. ( 2005; ). Salinivibrio costicola subsp. alcaliphilus subsp. nov., a haloalkaliphilic aerobe from Campania Region (Italy). Syst Appl Microbiol 28, 34–42.[CrossRef]
    [Google Scholar]
  21. Saito, H. & Miura, K. I. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
     [Google Scholar]
  23. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Smith, F. B. ( 1938; ). An investigation of a taint in rib bones of bacon. The determination of halophilic vibrios (n. spp.). Proc R Soc Queensl 49, 29–52.
     [Google Scholar]
  25. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  26. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  27. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  28. Tanasupawat, S., Pakdeeto, A., Namwong, S., Thawai, C., Kudo, T. & Itoh, T. ( 2006; ). Lentibacillus halophilus sp. nov., from fish sauce in Thailand. Int J Syst Evol Microbiol 56, 1859–1863.[CrossRef]
    [Google Scholar]
  29. Tanasupawat, S., Namwong, S., Kudo, T. & Itoh, T. ( 2007; ). Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand. Int J Syst Evol Microbiol 57, 1413–1417.[CrossRef]
    [Google Scholar]
  30. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  31. Thornley, M. J. ( 1960; ). The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Microbiol 23, 37–52.
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.001768-0
Loading
Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand
Int J Syst Evol Microbiol 59, 880 (2009); https://doi.org/10.1099/ijs.0.001768-0
/content/journal/ijsem/10.1099/ijs.0.001768-0
/content/journal/ijsem/10.1099/ijs.0.001768-0
Loading

Data & Media loading...

Supplements

Maximum-parsimony phylogenetic tree showing the relationships between strain ND1-1 , species of the genus and related taxa based on 16S rRNA gene sequences. [ PDF] 41 KB

PDF

Maximum-likelihood phylogenetic tree showing the relationships between strain ND1-1 , species of the genus and related taxa based on 16S rRNA gene sequences. [ PDF] 42 KB

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error