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A Gram-negative, facultatively anaerobic, moderately halophilic bacterium, strain ND1-1T, was isolated from fermented fish (pla-ra) in Thailand. The cells were curved rods, motile and non-endospore-forming. The novel strain grew optimally at 37 °C, at pH 8 and in the presence of 9−10 % (w/v) NaCl. The predominant respiratory lipoquinone was Q-8. The major cellular fatty acids were C16 : 0 and C12 : 0. Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 49.0 mol%. Comparative 16S rRNA gene sequence analyses indicated that strain ND1-1T was closely related to Salinivibrio costicola, which comprises three subspecies, and Salinivibrio proteolyticus with gene sequence similarities of 98.3–98.6 %. Strain ND1-1T showed low levels of DNA–DNA relatedness with S. costicola subsp. costicola JCM 15095T (33.2 %), S. costicola subsp. alcaliphilus DSM 16359T (38.4 %), S. costicola subsp. vallismortis JCM 15096T (59.7 %), and S. proteolyticus AF-2004T (42.1 %). On the basis of the physiological and biochemical characteristics and the molecular data presented, strain ND1-1T should be classified as a novel species of the genus Salinivibrio for which the name Salinivibrio siamensis sp. nov. is proposed. The type strain is ND1-1T (=JCM 14472T=PCU 301T=TISTR 1810T).
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