Gram-positive, non-spore-forming rods (strain 4401292) were isolated from a human blood sample. Based on cellular morphology and the results of biochemical tests, this strain was tentatively identified as belonging to an undescribed species of the genus . Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that the bacterium was related closely to (95.1 % 16S rRNA gene sequence similarity), (95.2 %), (95.2 %), (93.3 %) and (91.4 %). The predominant fatty acids were C18 : 19 and C16 : 0. On the basis of phenotypic data and phylogenetic inference, the novel species sp. nov. is proposed; the type strain is 4401292 (=CSUR P18=CCUG 53522).


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  1. An, D., Cai, S. & Dong, X.(2006).Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 56, 2043–2048.[CrossRef] [Google Scholar]
  2. Clarridge, J. E., III & Zhang, Q.(2002). Genotypic diversity of clinical Actinomyces species: phenotype, source, and disease correlation among genospecies. J Clin Microbiol 40, 3442–3448.[CrossRef] [Google Scholar]
  3. Hall, V., Talbot, P. R., Stubbs, S. L. & Duerden, B. I.(2001). Identification of clinical isolates of Actinomyces species by amplified 16S ribosomal DNA restriction analysis. J Clin Microbiol 39, 3555–3562.[CrossRef] [Google Scholar]
  4. Hall, V., Collins, M. D., Hutson, R. A., Inganas, E., Falsen, E. & Duerden, B. I.(2003a).Actinomyces oricola sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 53, 1515–1518.[CrossRef] [Google Scholar]
  5. Hall, V., Collins, M. D., Lawson, P. A., Falsen, E. & Duerden, B. I.(2003b).Actinomyces nasicola sp. nov., isolated from a human nose. Int J Syst Evol Microbiol 53, 1445–1448.[CrossRef] [Google Scholar]
  6. Hall, V., Collins, M. D., Lawson, P. A., Falsen, E. & Duerden, B. I.(2005).Actinomyces dentalis sp. nov., from a human dental abscess. Int J Syst Evol Microbiol 55, 427–431.[CrossRef] [Google Scholar]
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Miller, P. H., Wiggs, L. S. & Miller, J. M.(1995). Evaluation of API An-IDENT and RapID ANA II systems for identification of Actinomyces species from clinical specimens. J Clin Microbiol 33, 329–330. [Google Scholar]
  9. Saitou, N. & Nei, M.(1987). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  10. Santala, A. M., Sarkonen, N., Hall, V., Carlson, P., Jousimies-Somer, H. & Kononen, E.(2004). Evaluation of four commercial test systems for identification of Actinomyces and some closely related species. J Clin Microbiol 42, 418–420.[CrossRef] [Google Scholar]
  11. Sarkonen, N., Kononen, E., Summanen, P., Kononen, M. & Jousimies-Somer, H.(2001). Phenotypic identification of Actinomyces and related species isolated from human sources. J Clin Microbiol 39, 3955–3961.[CrossRef] [Google Scholar]
  12. Song, Y.(2005). PCR-based diagnostics for anaerobic infections. Anaerobe 11, 79–91.[CrossRef] [Google Scholar]
  13. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rossello-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  14. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  15. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]
  16. Woo, P. C., Ng, K. H. L., Lau, S. K. P., Yip, K. T., Fung, A. M. Y., Leung, K. W., Tam, D. M. W., Que, T. L. & Yuen, K. Y.(2003). Usefulness of the MicroSeq 500 16S ribosomal DNA-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. J Clin Microbiol 41, 1996–2001.[CrossRef] [Google Scholar]

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