1887

Abstract

A yellow-pigmented, moderately thermotolerant bacterial strain, designated S3-3, was isolated from desert sand in Xinjiang province, China, and subjected to a polyphasic taxonomic characterization. This isolate was Gram-negative, aerobic and motile. Phylogenetic analyses based on the 16S rRNA gene sequence showed that strain S3-3 is phylogenetically affiliated to the genus . Strain S3-3 exhibited levels of 16S rRNA gene sequence similarity of less than 96.3 % to the type strains of all species, making it clear that strain S3-3 represents a species that is separate from previously recognized species. Its major fatty acids were summed feature 8 (C 6 and/or C 7), summed feature 3 (C 7 and/or iso-C 2-OH) and C cyclo 8. The major isoprenoid quinone was Q-9. The DNA G+C content was 60.9 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic analysis, strain S3-3 should be classified within a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S3-3 (=CCTCC AB 207151 =NRRL B-51270).

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2009-06-01
2019-10-18
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References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Kawai, Y. & Yabuuchi, E. ( 1975; ). Pseudomonas pertucinogena sp. nov., an organism previously misidentified as Bordetella pertussis. Int J Syst Bacteriol 25, 317–323.[CrossRef]
    [Google Scholar]
  4. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  5. King, E. O., Ward, M. K. & Rainey, D. E. ( 1954; ). Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  6. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  7. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–176. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  8. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  9. MIDI ( 2005; ). Sherlock Microbial Identification System Operating Manual, version 6.0. Newark, DE: MIDI Inc.
  10. Migula, W. ( 1894; ). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238 (in German).
    [Google Scholar]
  11. Nishimori, E., Kita-Tsukamoto, K. & Wakabayshi, H. ( 2000; ). Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis. Int J Syst Evol Microbiol 50, 83–89.[CrossRef]
    [Google Scholar]
  12. Oyaizu, H. & Komagata, K. ( 1983; ). Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3-hydroxy fatty acids. J Gen Appl Microbiol 29, 17–40.[CrossRef]
    [Google Scholar]
  13. Palleroni, N. J. ( 1992; ). Introduction to the family Pseudomonadaceae. In The Prokaryotes, 2nd edn, pp. 3071–3085. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  14. Palleroni, N. J. ( 2005; ). Genus I. Pseudomonas Migula 1894, 237AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 323–379. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  15. Palleroni, N. J. & Doudoroff, M. ( 1972; ). Some properties and subdivisions of the genus Pseudomonas. Annu Rev Phytopathol 10, 73–100.[CrossRef]
    [Google Scholar]
  16. Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova, N. V. & Mikhailov, V. V. ( 2005; ). Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55, 919–924.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterisation. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Woods & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  21. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
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