A novel Gram-positive, aerobic, catalase-positive, filamentous micro-organism, designated strain IMMIB L-1269, originating from sputum was characterized using phenotypic and molecular taxonomic methods. It showed cell-wall chemotype III, phospholipid type PII (with phosphatidylethanolamine as the diagnostic phospholipid) and contained an unsaturated menaquinone with seven isoprene units (MK-7) as the predominant isoprenoid quinone. It synthesized long-chain cellular fatty acids of the straight-chain saturated, monounsaturated and iso- and anteiso-branched types (with iso-C, C and iso-C predominating) and possessed a DNA G+C content of 49.3 mol%. On the basis of its morphological, biochemical and chemical characteristics, strain IMMIB L-1269 did not conform to any presently recognized taxon. Comparative analyses based on 16S rRNA gene sequences confirmed the distinctiveness of the isolate, as it displayed sequence-divergence values greater than 7.7 % with respect to recognized Gram-positive taxa. Phylogenetic treeing analysis served to reinforce the view that strain IMMIB L-1269 was distinct from recognized taxa, as it formed a relatively long subline branching within a 16S rRNA gene sequence cluster that encompassed the genera , , , , , and of the family . On the basis of phenotypic and molecular phylogenetic evidence, strain IMMIB L-1269 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain IMMIB L-1269 (=DSM 45169 =CCUG 55916). An emended description of the family is also given.


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Straight to flexuous aerial mycelium of strain IMMIB L-1269 with endospores on Columbia agar after 18 h at 42 °C. Original magnification, ×800.


Scanning electron micrographs of strain IMMIB L-1269 grown for 3 days on yeast extract/malt extract agar at 42 °C. Note that aerial mycelium bears chains of arthrospores. Bars, 5 µm (top) and 1 µm (bottom).



Two-dimensional TLC of polar lipids of strain IMMIB L-1269 . DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL1, PL2, unidentified phospholipids; PME, phosphatidylmonomethylethanolamine; ?, unidentified component.


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