A polyphasic study was undertaken to clarify the taxonomic position of -like strains isolated from shellfish and humans. The diversity within the strain collection was initially screened by means of fluorescent amplified fragment length polymorphism analysis and whole-cell protein electrophoresis, revealing the existence of two clusters distinct from and other species. The divergence of these clusters was confirmed by phenotypic analysis and by 16S rRNA and gene sequence analysis. Phylogenetic analysis identified , , and as the closest phylogenetic neighbours of both taxa. DNA–DNA hybridizations revealed that one cluster, comprising 10 strains, represented a novel species, for which the name sp. nov. is proposed, with 2314BVA (=LMG 23910 =CCUG 55787) as the type strain. The second cluster, comprising six strains, represents a novel subspecies within the species , for which the name subsp. subsp. nov. is proposed, with 2897R (=LMG 21009 =CCUG 55786) as the type strain. The description of subsp. has the effect of automatically creating the subspecies subsp. subsp. nov. (type strain LMG 8846=NCTC 11352).


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vol. , part 5, pp. 1126 - 1132

Strain collection used in this study

Phylogenetic tree of selected strains based on gene sequences constructed by the neighbour-joining method

[PDF file of Supplementary Table S1 and Supplementary Fig. S1](351 KB)


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