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A novel Gram-positive, motile, rod-shaped bacterium isolated from a saline soil in China was characterized by a polyphasic taxonomic approach. The strain, designated YC1T, was halotolerant [tolerating up to 15 % (w/v) NaCl] and alkaliphilic (growing at a broad pH range of 5–13). 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Bacillus, showing highest similarity to Bacillus macauensis JCM 13285T (98.0 %). However, DNA–DNA hybridization indicated low levels of genomic relatedness with B. macauensis JCM 13285T (8.5 %). The major isoprenoid quinone was MK-7 and the cellular fatty acid profile consisted of significant amounts of iso-C15 : 0 (38.6 %) and anteiso-C15 : 0 (35.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the genomic DNA was 41.8 mol%. On the basis of the polyphasic evidence from this study, strain YC1T (=KCTC 13181T=CGMCC 1.6854T) should be classified as the type strain of a novel species of the genus Bacillus, for which the name Bacillus solisalsi sp. nov. is proposed.
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 6, pp. 1460 - 1464
Supplementary Fig. S1. Two-dimensional TLC of the polar lipids of strain YC1 Tand B. macauensis JCM 13285 T.
Supplementary Fig. S2. Transmission electron micrograph of cells of strain YC1 Tgrown on LB agar for 24 h at 30°C.
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