1887

Abstract

Four rhizobia-like strains, isolated from root nodules of and grown in Anhui and Jiangxi Provinces of China, were grouped into the genus but were distinct from all recognized species of the genus by phylogenetic analysis of 16S rRNA and housekeeping genes. The combined sequences of the housekeeping genes , and for strain CCBAU 23252 showed 86.9 to 95 % similarity to those of known species of the genus . All four strains had and genes and could form effective nodules with and and ineffective nodules with , but did not nodulate or in cross-nodulation tests. Fatty acid composition, DNA–DNA relatedness and a series of phenotypic tests also separated these strains from members of closely related species. Based on all the evidence, we propose a novel species, sp. nov., and designate CCBAU 23252 ( = CGMCC 1.12621 = LMG 27729) as the type strain. This strain was isolated from a root nodule of and has a DNA G+C content of 61.1 mol% ( ).

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2015-09-01
2019-10-14
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References

  1. Bond D.A. . ( 1976;). Field bean (Vicia faba). . In Evolution of Crop Plants, pp. 179–182. Edited by Simmonds N. W. . London: Longmans;.
    [Google Scholar]
  2. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12: 133–142 [CrossRef] [PubMed].
    [Google Scholar]
  3. Gao J.L. , Sun J.G. , Li Y. , Wang E.T. , Chen W.X. . ( 1994;). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44: 151–158 [CrossRef].
    [Google Scholar]
  4. George R.A.T. . ( 1999;). Vegetable Seed Production , 2nd edn.., Wallingford: CABI Publishing;.
    [Google Scholar]
  5. Graham P.H. , Sadowsky M.J. , Keyser H.H. , Barnet Y.M. , Bradley R.S. , Cooper J.E. , De Ley D.J. , Jarvis B.D.W. , Roslycky E.B. , other authors . ( 1991;). Proposed minimal standards for the description of new genera and species of root-and stem-nodulating bacteria. Int J Syst Bacteriol 41: 582–587.[CrossRef]
    [Google Scholar]
  6. Jiao Y.S. , Yan H. , Ji Z.J. , Liu Y.H. , Sui X.H. , Wang E.T. , Guo B.L. , Chen W.X. , Chen W.F. . ( 2015;). Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of the medicinal legume Sophora flavescens. Int J Syst Evol Microbiol 65: 497–503.[CrossRef]
    [Google Scholar]
  7. Kurtz S. , Phillippy A. , Delcher A.L. , Smoot M. , Shumway M. , Antonescu C. , Salzberg S.L. . ( 2004;). Versatile and open software for comparing large genomes. Genome Biol 5: R12 [CrossRef] [PubMed].
    [Google Scholar]
  8. Laguerre G. , Nour S.M. , Macheret V. , Sanjuan J. , Drouin P. , Amarger N. . ( 2001;). Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts. Microbiology 147: 981–993 [PubMed].[CrossRef]
    [Google Scholar]
  9. Laguerre G. , Louvrier P. , Allard M.R. , Amarger N. . ( 2003;). Compatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum biovar viciae for nodulation of host legumes. Appl Environ Microbiol 69: 2276–2283 [CrossRef] [PubMed].
    [Google Scholar]
  10. Mutch L.A. , Young J.P.W. . ( 2004;). Diversity and specificity of Rhizobium leguminosarum biovar viciae on wild and cultivated legumes. Mol Ecol 13: 2435–2444 [CrossRef] [PubMed].
    [Google Scholar]
  11. Ramírez-Bahena M.H. , García-Fraile P. , Peix A. , Valverde A. , Rivas R. , Igual J.M. , Mateos P.F. , Martínez-Molina E. , Velázquez E. . ( 2008;). Revision of the taxonomic status of the species Rhizobium leguminosarum (Frank 1879) Frank 1889AL Rhizobium phaseoli Dangeard 1926AL Rhizobium trifolii Dangeard 1926AL R. trifolii is a later synonym of R. leguminosarum. Reclassification of the strain R. leguminosarum DSM 30132 ( = NCIMB 11478) as Rhizobium pisi sp. nov. Int J Syst Evol Microbiol 58: 2484–2490 [CrossRef] [PubMed].
    [Google Scholar]
  12. Richter M. , Rosselló-Móra R. . ( 2009;). Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 106: 19126–19131 [CrossRef] [PubMed].
    [Google Scholar]
  13. Saïdi S. , Ramírez-Bahena M.H. , Santillana N. , Zúñiga D. , Álvarez-Martínez E. , Peix A. , Mhamdi R. , Velázquez E. . ( 2014;). Rhizobium laguerreae sp. nov. nodulates Vicia faba on several continents. Int J Syst Evol Microbiol 64: 242–247 [CrossRef] [PubMed].
    [Google Scholar]
  14. Sarita S. , Sharma P.K. , Priefer U.B. , Prell J. . ( 2005;). Direct amplification of rhizobial nodC sequences from soil total DNA and comparison to nodC diversity of root nodule isolates. FEMS Microbiol Ecol 54: 1–11 [CrossRef] [PubMed].
    [Google Scholar]
  15. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: Microbial MIDI Inc;.
    [Google Scholar]
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  17. Tan Z.Y. , Xu X.D. , Wang E.T. , Gao J.L. , Martinez-Romero E. , Chen W.X. . ( 1997;). Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. Int J Syst Bacteriol 47: 874–879 [CrossRef] [PubMed].
    [Google Scholar]
  18. Tian C.F. , Wang E.T. , Wu L.J. , Han T.X. , Chen W.F. , Gu C.T. , Gu J.G. , Chen W.X. . ( 2008;). Rhizobium fabae sp. nov., a bacterium that nodulates Vicia faba . Int J Syst Evol Microbiol 58: 2871–2875 [CrossRef] [PubMed].
    [Google Scholar]
  19. Tighe S.W. , de Lajudie P. , Dipietro K. , Lindström K. , Nick G. , Jarvis B.D.W. . ( 2000;). Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50: 787–801 [CrossRef] [PubMed].
    [Google Scholar]
  20. Versalovic J. , Schneider M. , de Bruijn F.J. , Lupski J.R. . ( 1994;). Genomic fingerprinting of bacteria using repetitive sequence based PCR (rep-PCR). Methods Mol Cell Biol 5: 25–40.
    [Google Scholar]
  21. Vincent J.M. . ( 1970;). A Manual for the Practical Study of the Root Nodule Bacteria., Oxford: Blackwell Scientific;.
    [Google Scholar]
  22. Vinuesa P. , Silva C. , Werner D. , Martínez-Romero E. . ( 2005;). Population genetics and phylogenetic inference in bacterial molecular systematics: the roles of migration and recombination in Bradyrhizobium species cohesion and delineation. Mol Phylogenet Evol 34: 29–54 [CrossRef] [PubMed].
    [Google Scholar]
  23. Wang R. , Chang Y.L. , Zheng W.T. , Zhang D. , Zhang X.X. , Sui X.H. , Wang E.T. , Hu J.Q. , Zhang L.Y. , Chen W.X. . ( 2013;). Bradyrhizobium arachidis sp. nov., isolated from effective nodules of Arachis hypogaea grown in China. Syst Appl Microbiol 36: 101–105 [CrossRef] [PubMed].
    [Google Scholar]
  24. Young J.M. , Kuykendall L.D. , Martínez-Romero E. , Kerr A. , Sawada H. . ( 2001;). A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis . Int J Syst Evol Microbiol 51: 89–103 [PubMed].[CrossRef]
    [Google Scholar]
  25. Zheng W.T. , Li Y. Jr , Wang R. , Sui X.H. , Zhang X.X. , Zhang J.J. , Wang E.T. , Chen W.X. . ( 2013;). Mesorhizobium qingshengii sp. nov., isolated from effective nodules of Astragalus sinicus . Int J Syst Evol Microbiol 63: 2002–2007 [CrossRef] [PubMed].
    [Google Scholar]
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