1887

Abstract

A bacterial strain designated 11N27 was isolated from an agricultural soil sample. Cells of this strain were Gram-reaction-variable, facultatively anaerobic, endospore-forming, white-pigmented, peritrichously flagellated and hydrolysed xanthine. The major fatty acids of strain 11N27 were anteiso-C, iso-C and C. The polar lipid profile contained phosphatidylethanolamine, two unknown phospholipids, two unknown aminolipids, one unknown aminophospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain 11N27 was 50.3 mol%. MK-7 was the predominant respiratory quinone. -Diaminopimelic acid was the diagnostic diamino acid in the peptidoglycan. 16S rRNA gene sequence analysis showed that strain 11N27 was phylogenetically related to C/2 (96.2  % sequence similarity) and CMM 7311 (96.0  %). The genotypic and phenotypic data showed that strain 11N27 could be distinguished from phylogenetically related species and that this strain represents a novel species of the genus . The name sp. nov. is proposed with the type strain 11N27( = KACC 17935 = NBRC 109108).

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2015-09-01
2019-10-14
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References

  1. Ash C. , Priest F.G. , Collins M.D. . ( 1993;). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253–260 [CrossRef] [PubMed].
    [Google Scholar]
  2. Baik K.S. , Lim C.H. , Choe H.N. , Kim E.M. , Seong C.N. . ( 2011;). Paenibacillus rigui sp. nov., isolated from a freshwater wetland. Int J Syst Evol Microbiol 61: 529–534.[CrossRef]
    [Google Scholar]
  3. Baker G.C. , Smith J.J. , Cowan D.A. . ( 2003;). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55: 541–555.[CrossRef]
    [Google Scholar]
  4. Counsell T.J. , Murray R.G.E. . ( 1986;). Polar lipid profiles of the genus Deinococcus . Int J Syst Bacteriol 36: 202–206 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W.M. . ( 1971;). Toward defining course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef]
    [Google Scholar]
  7. Glaeser S.P. , Falsen E. , Busse H.J. , Kämpfer P. . ( 2013;). Paenibacillus vulneris sp. nov., isolated from a necrotic wound. Int J Syst Evol Microbiol 63: 777–782 [CrossRef] [PubMed].
    [Google Scholar]
  8. Jukes T.H. , Cantor C.R. . ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism vol. 3, pp. 21–132. Edited by Munro H. N. . New York: Academic Press; [CrossRef]
    [Google Scholar]
  9. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  10. Komagata K. , Suzuki K. . ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207.[CrossRef]
    [Google Scholar]
  11. Lane D.J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . Chichester: Wiley;.
    [Google Scholar]
  12. Lee J.C. , Yoon K.H. . ( 2008;). Paenibacillus woosongensis sp. nov., a xylanolytic bacterium isolated from forest soil. Int J Syst Evol Microbiol 58: 612–616 [CrossRef] [PubMed].
    [Google Scholar]
  13. Lee S. , Malone C. , Kemp P. . ( 1993;). Use of multiple 16S rRNA-targeted fluorescent probes to increase signal strength and measure cellular RNA from natural planktonic bacteria. Mar Ecol Prog Ser 101: 193–201 [CrossRef]
    [Google Scholar]
  14. Logan N.A. , Berge O. , Bishop A.H. , Busse H.J. , De Vos P. , Fritze D. , Heyndrickx M. , Kämpfer P. , Rabinovitch L. , other authors . ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59: 2114–2121 [CrossRef] [PubMed].
    [Google Scholar]
  15. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  16. McSpadden Gardener B.B. . ( 2004;). Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathology 94: 1252–1258 [CrossRef] [PubMed].
    [Google Scholar]
  17. MIDI ( 1999;). Sherlock Microbial Identification System. Operating Manual, version 3.0. ., Newark, DE: MIDI Inc;.
  18. Nelson D.M. , Glawe A.J. , Labeda D.P. , Cann I.K. , Mackie R.I. . ( 2009;). Paenibacillus tundrae sp. nov. and Paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from Alaskan tundra. Int J Syst Evol Microbiol 59: 1708–1714.[CrossRef]
    [Google Scholar]
  19. Rivas R. , Mateos P.F. , Martínez-Molina E. , Velázquez E. . ( 2005;). Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55: 405–408 [CrossRef] [PubMed].
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425.
    [Google Scholar]
  21. Shida O. , Takagi H. , Kadowaki K. , Nakamura L.K. , Komagata K. . ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47: 289–298.[CrossRef]
    [Google Scholar]
  22. Smibert R.M. , Krieg N.R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  23. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  24. Thompson J.D. , Higgins D.G. , Gibson T.J. . ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680 [CrossRef] [PubMed].
    [Google Scholar]
  25. von der Weid I. , Duarte G.F. , van Elsas J.D. , Seldin L. . ( 2002;). Paenibacillus brasilensis sp. nov., a novel nitrogen-fixing species isolated from the maize rhizosphere in Brazil. Int J Syst Evol Microbiol 52: 2147–2153 [CrossRef] [PubMed].
    [Google Scholar]
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