1887

Abstract

A Gram-stain-negative, yellow-pigmented, aerobic, non-flagellated, non-gliding bacterial strain, designated SM1203, was isolated from surface seawater of Kongsfjorden, Svalbard. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1203 was affiliated with the genus in the family . The strain shared the highest 16S rRNA gene sequence similarity (>96  %) with the type strains of (96.8  %), (96.3  %), (96.3  %) and (96.1  %) and 95.4–95.7  % sequence similarity with the type strains of other known species of the genus . The strain grew at 4–30 °C and in the presence of 1.0–5.0  % (w/v) NaCl. The major fatty acids of strain SM1203 were iso-C, iso-C, anteiso-C and C and the main polar lipids were phosphatidylethanolamine and an unidentified lipid. The major respiratory quinone of strain SM1203 was menaquinone 6 (MK-6). The genomic DNA G+C content of strain SM1203 was 34.8 mol%. Based on the polyphasic characterization of strain SM1203 in this study, the strain represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SM1203 ( = CGMCC 1.12751 = JCM 30333). An emended description of the genus is also given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000357
2015-09-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/2925.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000357&mimeType=html&fmt=ahah

References

  1. Bercovich A., Vazquez S.C., Yankilevich P., Coria S.H., Foti M., Hernández E., Vidal A., Ruberto L., Melo C., other authors. ( 2008;). Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica. Int J Syst Evol Microbiol 58 23632367 [View Article] [PubMed] .
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [PubMed].
    [Google Scholar]
  3. Bowman J.P., Nichols D.S. ( 2005;). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55 14711486 [View Article] [PubMed] .
    [Google Scholar]
  4. Collins M.D., Jones D. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48 459470 [View Article].
    [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed] .
    [Google Scholar]
  6. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  7. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  8. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  9. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [PubMed]. [CrossRef]
    [Google Scholar]
  10. Kobayashi T., Imada C., Hiraishi A., Tsujibo H., Miyamoto K., Inamori Y., Hamada N., Watanabe E. ( 2003;). Pseudoalteromonas sagamiensis sp. nov., a marine bacterium that produces protease inhibitors. Int J Syst Evol Microbiol 53 18071811 [View Article] [PubMed] .
    [Google Scholar]
  11. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161207. [CrossRef]
    [Google Scholar]
  12. Lane D.J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  13. Lee S.-Y., Park S., Oh T.-K., Yoon J.-H. ( 2012;). Salinimicrobium gaetbulicola sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 62 10271031 [View Article] [PubMed] .
    [Google Scholar]
  14. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3 208218. [CrossRef]
    [Google Scholar]
  15. Marmur J., Doty P. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5 109118 [PubMed]. [CrossRef]
    [Google Scholar]
  16. Murray R.G.E., Doetsch R.N., Robinow C.F. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  17. Nedashkovskaya O.I., Kim S.B., Lysenko A.M., Frolova G.M., Mikhailov V.V., Bae K.S. ( 2005;). Bizionia paragorgiae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the soft coral Paragorgia arborea . Int J Syst Evol Microbiol 55 375378 [PubMed]. [CrossRef]
    [Google Scholar]
  18. Nedashkovskaya O.I., Vancanneyt M., Kim S.B. ( 2010;). Bizionia echini sp. nov., isolated from a sea urchin. Int J Syst Evol Microbiol 60 928931 [View Article] [PubMed] .
    [Google Scholar]
  19. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  20. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  21. Song E.-J., Lee M.-H., Seo M.-J., Yim K.J., Hyun D.-W., Bae J.-W., Park S.L., Roh S.W., Nam Y.-D. ( 2014;). Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 106 837844 [View Article] [PubMed] .
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  23. Yoon B.-J., Oh D.-C. ( 2011;). Formosa spongicola sp. nov., isolated from the marine sponge Hymeniacidon flavia . Int J Syst Evol Microbiol 61 330333 [View Article] [PubMed] .
    [Google Scholar]
  24. Yoon J.-H., Kang C.-H., Jung Y.-T., Kang S.-J. ( 2013;). Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm. Int J Syst Evol Microbiol 63 685690 [View Article] [PubMed] .
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.000357
Loading
/content/journal/ijsem/10.1099/ijs.0.000357
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error