1887

Abstract

A Gram-stain-negative, yellow, rod-shaped bacterium, designated strain KK22, was isolated from a microbial consortium that grew on diesel fuel originally recovered from cattle pasture soil. Strain KK22 has been studied for its ability to biotransform high molecular weight polycyclic aromatic hydrocarbons. On the basis of 16S rRNA gene sequence phylogeny, strain KK22 was affiliated with the genus in the phylum and was most closely related to TKP (99.8  %) and less closely related to P25 (97.5  %). Results of DNA–DNA hybridization (DDH) revealed relatedness values between strain KK22 and strain TKP and between strain KK22 and strain P25 of 21 ± 4  % (reciprocal hybridization, 27 ± 2  %) and 15 ± 2  % (reciprocal hybridization, 17 ± 1  %), respectively. Chemotaxonomic analyses of strain KK22 showed that the major respiratory quinone was ubiquinone Q-10, that the polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidyl--methylethylethanolamine and sphingoglycolipid, and that Cω7 and C 2-OH were the main fatty acid and hydroxylated fatty acids, respectively. This strain was unable to reduce nitrate and the genomic DNA G+C content was 64.7 mol%. Based upon the results of the DDH analyses, the fact that strain KK22 was motile, and its biochemical and physiological characteristics, strain KK22 could be separated from recognized species of the genus . We conclude that strain KK22 represents a novel species of this genus for which the name sp. nov. is proposed; the type strain is KK22 ( = DSM 29313 = JCM 30309).

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2015-09-01
2019-09-22
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