1887

Abstract

Four isolates of an unknown Gram-stain-positive, catalase-negative coccus-shaped organism, isolated from the pharynx of four wild rabbits, were characterized by phenotypic and molecular genetic methods. The micro-organisms were tentatively assigned to the genus based on cellular morphological and biochemical criteria, although the organisms did not appear to correspond to any species with a validly published name. Comparative 16S rRNA gene sequencing confirmed their identification as members of the genus , being most closely related phylogenetically to 682-03 (96.9  % 16S rRNA gene sequence similarity). Analysis of and gene sequences showed divergence values between the novel species and 682-03 (the closest phylogenetic relative determined from 16S rRNA gene sequences) of 18.1 and 23.9  %, respectively. The novel bacterial isolate could be distinguished from the type strain of by several biochemical characteristics, such as the production of glycyl-tryptophan arylamidase and α-chymotrypsin, and the non-acidification of different sugars. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be assigned to a novel species of the genus , and named sp. nov. The type strain is DICM10-00796B ( = CECT 8754 = CCUG 66496).

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2015-09-01
2021-10-23
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