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Abstract

Two strictly anaerobic bacterial strains, designated SW512 and W72, were isolated from a laboratory-scale H-producing up-flow anaerobic sludge blanket (UASB) reactor. Cells were Gram-stain-negative, non-motile and 0.3–0.4×2.0–14.5 μm; they did not form spores. Both strains grew at 24–45 °C (no growth at ≤22 °C or ≥46 °C), with optimum growth at 37 °C. The pH range for growth was 4.5–9.0 (no growth at pH≤4.2 or pH≥9.3), with optimum growth at pH 6.5–7.0. Several kinds of mono-, di- and oligosaccharides supported growth. The main end products of glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide (according to the equation 1 mol glucose→1.1 mol acetate+0.6 mol ethanol+2.6 mol H+1.6 mol CO). The DNA G+C contents of strains SW512 and W72 were 41.9±0.5 and 42.8±0.4 mol% ( method), respectively. Phylogenetic analysis based on 16S rRNA gene sequence similarities indicated that the two strains represent a new phyletic sublineage within the family ‘’, with <91.3 % 16S rRNA gene sequence similarity to recognized species. On the basis of the polyphasic evidence in this study, it is proposed that the two strains represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain of the type species is SW512 (=AS 1.5070=JCM 14861).

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2009-02-01
2021-07-30
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vol. , part 2, pp. 295 - 299

Phylogenetic dendrogram of sp. nov. SW512 and related species based on 16S rRNA gene similarity. [ PDF] 37 KB



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