1887

Abstract

A staphylocoagulase-negative, novobiocin-susceptible strain (M4S-6) of a species of the genus was isolated from the river Argen in Southern Germany. It was assigned to the genus due to the presence of the fatty acids, ai-C, i-C, i-C, ai-C, and of menaquinone (MK-7) in the cytoplasmic membrane, which are typical of coagulase-negative staphylococci. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. Although the 16S gene sequence of strain M4S-6 revealed a 98 % similarity with its closest relative, , it could be distinguished by several phenotypical and physiological markers. In contrast to , M4S-6 was ornithine decarboxylase-positive, urease-negative and could use formiate and -histidine as carbon-sources; nitrate was not reduced. Whereas could grow with (-)-mannitol, -sorbitol, gluconic acid, -proline, carboxymethylcellulose and lignosulfonate, M4S-6 was not able to grow with these substances. The results of 16S rRNA gene sequence analysis and of phenotypic testing indicated that M4S-6 was a representative of a novel species for which the name sp. nov., is proposed with the type strain M4S-6 (DSM 29875 = CIP 110904).

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2015-08-01
2019-10-23
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