1887

Abstract

An actinomycete strain, designated YIM 690008, was isolated from collected from South Korea and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. The whole-cell hydrolysates contained -diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. The predominant menaquinones were MK-10(H), MK-10(H), MK-10(H) and MK-10(H). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides, some unknown phospholipids, glycolipids and polar lipids. The major fatty acids were i-C, ai-C and Cω9. The DNA G+C content of the genomic DNA was 63.1 mol%. Phylogenetic analysis indicated that the isolate belongs to the family . However, based on phenotypic, chemotaxonomic and genotypic data, it was concluded that strain YIM 690008 represents a novel genus and novel species of the family , for which the name gen. nov., sp. nov. (type strain YIM 690008 = DSM 46770 = JCM 30099 = KCTC 29480) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000304
2015-08-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2562.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000304&mimeType=html&fmt=ahah

References

  1. Cai M. , Zhi X.Y. , Tang S.K. , Zhang Y.Q. , Xu L.H. , Li W.J. . ( 2008;). Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. Int J Syst Evol Microbiol 58: 1556–1560 [CrossRef] [PubMed].
    [Google Scholar]
  2. Chang X. , Liu W. , Zhang X.-H. . ( 2011;). Spinactinospora alkalitolerans gen. nov., sp. nov., an actinomycete isolated from marine sediment. Int J Syst Evol Microbiol 61: 2805–2810 [CrossRef] [PubMed].
    [Google Scholar]
  3. Chang X. , Liu W. , Zhang X.H. . ( 2012;). Salinactinospora qingdaonensis gen. nov., sp. nov., a halophilic actinomycete isolated from a salt pond. Int J Syst Evol Microbiol 62: 954–959 [CrossRef] [PubMed].
    [Google Scholar]
  4. Chen Y.G. , Wang Y.X. , Zhang Y.Q. , Tang S.K. , Liu Z.X. , Xiao H.D. , Xu L.H. , Cui X.L. , Li W.J. . ( 2009;). Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59: 2708–2713 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M.D. , Jones D. . ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48: 459–470 [CrossRef].
    [Google Scholar]
  6. Collins M.D. , Pirouz T. , Goodfellow M. , Minnikin D.E. . ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  7. Cui X.-L. , Mao P.-H. , Zeng M. , Li W.-J. , Zhang L.-P. , Xu L.-H. , Jiang C.-L. . ( 2001;). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51: 357–363 [PubMed].[CrossRef]
    [Google Scholar]
  8. Dong X.Z. , Cai M.Y. . ( 2001;). Manual of Systematics and Identification of General Bacteria Beijing: Science Press;.
    [Google Scholar]
  9. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  10. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  11. Fitch W.M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  12. Gordon R.E. , Barnett D.A. , Handerhan J.E. , Pang C.H.-N. . ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24: 54–63 [CrossRef].
    [Google Scholar]
  13. Groth I. , Rodríguez C. , Schütze B. , Schmitz P. , Leistner E. , Goodfellow M. . ( 2004;). Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54: 2121–2129 [CrossRef] [PubMed].
    [Google Scholar]
  14. Guo L. , Tuo L. , Habden X. , Zhang Y. , Liu J. , Jiang Z. , Liu S. , Dilbar T. , Sun C. . ( 2015;). Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. Int J Syst Evol Microbiol 65: 206–213 [CrossRef] [PubMed].
    [Google Scholar]
  15. Hasegawa T. , Takizawa M. , Tanida S. . ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29: 319–322 [CrossRef].
    [Google Scholar]
  16. Jiang Y. , Tang S.K. , Wiese J. , Xu L.H. , Imhoff J.F. , Jiang C.L. . ( 2007;). Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces . Int J Syst Evol Microbiol 57: 2694–2698 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kämpfer P. , Schäfer J. , Lodders N. , Martin K. . ( 2010;). Murinocardiopsis flavida gen. nov., sp. nov., an actinomycete isolated from indoor walls. Int J Syst Evol Microbiol 60: 1729–1734 [CrossRef] [PubMed].
    [Google Scholar]
  18. Kelly K.L. . ( 1964;). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;.
    [Google Scholar]
  19. Kim O.S. , Cho Y.J. , Lee K. , Yoon S.H. , Kim M. , Na H. , Park S.C. , Jeon Y.S. , Lee J.H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  20. Kroppenstedt R.M. , Evtushenko L.I. . ( 2006;). The family Nocardiopsaceae . . In The Prokaryotes: A Handbook on the Biology of Bacteria , 3rd edn. vol. 3, pp. 754–795. Edited by Dworkin M , Falkow S , Rosenberg E , Schleifer K. H , Stackebrandt E . New York: Springer-Verlag; [CrossRef].
    [Google Scholar]
  21. Lechevalier M.P. , Lechevalier H.A. . ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20: 435–443 [CrossRef].
    [Google Scholar]
  22. Leifson E. . ( 1960;). Atlas of Bacterial Flagellation London: Academic Press;.[CrossRef]
    [Google Scholar]
  23. Li W.J. , Xu P. , Schumann P. , Zhang Y.Q. , Pukall R. , Xu L.H. , Stackebrandt E. , Jiang C.L. . ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57: 1424–1428 [CrossRef] [PubMed].
    [Google Scholar]
  24. Li J. , Zhao G.Z. , Chen H.H. , Wang H.B. , Qin S. , Zhu W.Y. , Xu L.H. , Jiang C.L. , Li W.J. . ( 2008;). Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest. Lett Appl Microbiol 47: 574–580 [CrossRef] [PubMed].
    [Google Scholar]
  25. Mesbah M. , Premachandran U. , Whitman W.B. . ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  26. Meyer J. . ( 1976;). Nocardiopsis, a new genus of the order Actinomycetales . Int J Syst Bacteriol 26: 487–493 [CrossRef].
    [Google Scholar]
  27. Minnikin D.E. , Collins M.D. , Goodfellow M. . ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47: 87–95 [CrossRef].
    [Google Scholar]
  28. Rainey F.A. , Ward-Rainey N. , Kroppenstedt R.M. , Stackebrandt E. . ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46: 1088–1092 [CrossRef] [PubMed].
    [Google Scholar]
  29. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  30. Shirling E.B. , Gottlieb D. . ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16: 313–340 [CrossRef].
    [Google Scholar]
  31. Smibert R.M. , Krieg N.R. . ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriolog, pp. 607–654. Edited by Gerhardt P , Murray R. G. E , Wood W. A , Krieg N. R . Washington, D.C: American Society for Microbiology;.
    [Google Scholar]
  32. Tamaoka J. , Katayama-Fujimura Y. , Kuraishi H. . ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54: 31–36 [CrossRef].
    [Google Scholar]
  33. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  34. Tang S.-K. , Tian X.-P. , Zhi X.-Y. , Cai M. , Wu J.-Y. , Yang L.-L. , Xu L.-H. , Li W.-J. . ( 2008;). Haloactinospora alba gen. nov., sp. nov., a halophilic filamentous actinomycete of the family Nocardiopsaceae . Int J Syst Evol Microbiol 58: 2075–2080 [CrossRef] [PubMed].
    [Google Scholar]
  35. Tang S.K. , Wang Y. , Chen Y. , Lou K. , Cao L.L. , Xu L.H. , Li W.J. . ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59: 2025–2032 [CrossRef] [PubMed].
    [Google Scholar]
  36. Thompson J.D. , Gibson T.J. , Plewniak F. , Jeanmougin F. , Higgins D.G. . ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882 [CrossRef] [PubMed].
    [Google Scholar]
  37. Tian X.-P. , Tang S.-K. , Dong J.-D. , Zhang Y.-Q. , Xu L.-H. , Zhang S. , Li W.-J. . ( 2009;). Marinactinospora thermotolerans gen. nov., sp. nov., a marine actinomycete isolated from a sediment in the northern South China Sea. Int J Syst Evol Microbiol 59: 948–952 [CrossRef] [PubMed].
    [Google Scholar]
  38. Xu P. , Li W.J. , Tang S.K. , Zhang Y.Q. , Chen G.Z. , Chen H.H. , Xu L.H. , Jiang C.L. . ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55: 1149–1153 [CrossRef] [PubMed].
    [Google Scholar]
  39. Yang L.L. , Tang S.K. , Zhang Y.Q. , Zhi X.Y. , Wang D. , Xu L.H. , Li W.J. . ( 2008a;). Thermobifida halotolerans sp. nov., isolated from a salt mine sample, and emended description of the genus Thermobifida . Int J Syst Evol Microbiol 58: 1821–1825 [CrossRef] [PubMed].
    [Google Scholar]
  40. Yang R. , Zhang L.P. , Guo L.G. , Shi N. , Lu Z. , Zhang X. . ( 2008b;). Nocardiopsis valliformis sp. nov., an alkaliphilic actinomycete isolated from alkali lake soil in China. Int J Syst Evol Microbiol 58: 1542–1546 [CrossRef] [PubMed].
    [Google Scholar]
  41. Zhang Z. , Wang Y. , Ruan J. . ( 1998;). Reclassification of Thermo‐monospora Microtetraspora . Int J Syst Bacteriol 48: 411–422 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000304
Loading
/content/journal/ijsem/10.1099/ijs.0.000304
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error