1887

Abstract

An aerobic, Gram-stain-positive actinomycete, designated strain 3SM4-07, was characterized using a polyphasic taxonomic approach. The strain produced branching mycelium which fragmented into short or elongated rods. The whole-cell hydrolysates contained -2,6-diaminopimelic acid as the diagnostic diamino acid, with glucose and ribose as the main sugars. The predominant cellular fatty acids were anteiso-C, iso-C and iso-C.The predominant menaquinone was MK-9(H). Phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were absent. The DNA G+C content was 72.3 mol%. Strain 3SM4-07 formed a phylogenetic line within the genus and its 16S rRNA gene sequence was related most closely to D8-87 (99.0 % similarity), 15-Je-017 (98.8 %), YIM 61503 (98.6 %) and YIM 002 (98.6 %). However, mean DNA–DNA hybridization values revealed that strain 3SM4-07 differed from the closest species previously described in this genus. Data from phenotypic, chemotaxonomic and molecular analyses between strain 3SM4-07 and recognized species of the genus indicate that strain 3SM4-07 is a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3SM4-07 ( = BCC 60398 = NBRC 109648).

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2015-08-01
2021-03-01
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