1887

Abstract

Strain XMU 506, isolated from the rhizosphere soil of an ornamental plant, L., collected from Xiamen City, China, was identified using a polyphasic approach to clarify its taxonomic position. The aerial mycelium of this organism formed long straight or curved chains of spores and sporangium-like structures. The optimum growth occurred at 28–30 °C, pH 7.0 with 0–1 % NaCl. Strain XMU 506 showed the highest 16S rRNA gene sequence similarity (96.5  %) to DSM 44226, and formed a monophyletic clade in the 16S rRNA gene phylogenetic tree together with the type strains of the genus . The chemotaxonomic properties further supported the assignment of strain XMU 506 to the genus : -diaminopimelic acid was the diagnostic amino acid in the cell wall peptidoglycan; mycolic acids were not present in the cell wall; the whole-cell hydrolysates contained arabinose, galactose, glucose and ribose. The major menaquinone was MK-9(H); the phospholipids of the isolate comprised phosphatidylethanolamine, OH-phosphatidylethanolamine, diphosphatidylglycerol and unidentified amino-, glyco- and phospholipids; the major fatty acids of the strain were iso-C, C ω6 and iso-C H. The G+C content of genomic DNA was 67.3 mol%. Based on the results of phylogenetic analysis, phenotypic and genotypic characterization, strain XMU 506 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is XMU 506 ( = KCTC 29675 = MCCC 1K00430).

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2015-08-01
2021-04-23
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