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Abstract

Two bacterial strains (JC247 and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C, iso-C, iso-C, Cω11 and C, with minor ( < 5 but >1 %) amounts of anteiso-C, anteiso-C, iso-C H, iso-C, iso-C, C, C, C, Cω9, iso-Cω10 and anteiso-CB/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. The genomic DNA G+C content of strains JC247 and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA–DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus within the family of the phylum . Both strains had a 16S rRNA gene sequence similarity of 96.93 % with NCIMB 9366 and < 96.92 % with other members of the genus . Sequence similarity between strain JC247 and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247 and JC248 as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC247 ( = KCTC 33627 = LMG 28608).

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2015-08-01
2020-08-04
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