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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 65, Issue Pt_8

sp. nov., isolated from soil Free

Abstract

Two bacterial strains (JC247 and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C    , iso-C    , iso-C    , C    ω11 and C    , with minor ( < 5 but >1 %) amounts of anteiso-C    , anteiso-C    , iso-C    H, iso-C    , iso-C    , C    , C    , C    , C    ω9, iso-C    ω10 and anteiso-C    B/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. The genomic DNA G+C content of strains JC247 and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA–DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus within the family of the phylum . Both strains had a 16S rRNA gene sequence similarity of 96.93 % with NCIMB 9366 and < 96.92 % with other members of the genus . Sequence similarity between strain JC247 and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247 and JC248 as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC247 ( = KCTC 33627 = LMG 28608).

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2015-08-01
2025-05-16
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Bacillus crescens sp. nov., isolated from soil
Int J Syst Evol Microbiol 65, 2531 (2015); https://doi.org/10.1099/ijs.0.000296
/content/journal/ijsem/10.1099/ijs.0.000296
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