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Abstract

Two bacterial strains (JC247 and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C, iso-C, iso-C, Cω11 and C, with minor ( < 5 but >1 %) amounts of anteiso-C, anteiso-C, iso-C H, iso-C, iso-C, C, C, C, Cω9, iso-Cω10 and anteiso-CB/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. The genomic DNA G+C content of strains JC247 and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA–DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus within the family of the phylum . Both strains had a 16S rRNA gene sequence similarity of 96.93 % with NCIMB 9366 and < 96.92 % with other members of the genus . Sequence similarity between strain JC247 and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247 and JC248 as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC247 ( = KCTC 33627 = LMG 28608).

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2015-08-01
2025-05-16
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References

  1. Amoozegar M.A., Didari M., Bagheri M., Fazeli S.A.S.F., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013;). Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. Int J Syst Evol Microbiol 63 33243329 [View Article] [PubMed].
    [Google Scholar]
  2. Cappuccino J.G., Sherman N. ( 1998). Microbiology: a laboratory manual , 5th edition. Menlo Park, Ca: Benjamin/Cummings;.
    [Google Scholar]
  3. Carrasco I.J., Márquez M.C., Xue Y., Ma Y., Cowan D.A., Jones B.E., Grant W.D., Ventosa A. ( 2007;). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. Int J Syst Evol Microbiol 57 20842088 [View Article] [PubMed].
    [Google Scholar]
  4. Chakravarthy S.K., Ramaprasad E.V.V., Shobha E., Sasikala Ch., Ramana ChV. ( 2012;). Rhodoplanes piscinae sp. nov. isolated from pond water. Int J Syst Evol Microbiol 62 28282834 [View Article] [PubMed].
    [Google Scholar]
  5. Dastager S.G., Mawlankar R., Tang S.K., Srinivasan K., Ramana V.V., Shouche Y.S. ( 2014;). Bacillus enclensis sp. nov., isolated from sediment sample. Antonie van Leeuwenhoek 105 199206 [View Article] [PubMed].
    [Google Scholar]
  6. Edgar R.C. ( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32 17921797 [View Article] [PubMed].
    [Google Scholar]
  7. Hugh R., Leifson E. ( 1953;). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66 2426 [PubMed].
    [Google Scholar]
  8. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B.J. ( 2006;). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56 781786 [View Article] [PubMed].
    [Google Scholar]
  9. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  10. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  11. Kosowski K., Schmidt M., Pukall R., Hause G., Kämpfer P., Lechner U. ( 2014;). Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. Int J Syst Evol Microbiol 64 8894 [View Article] [PubMed].
    [Google Scholar]
  12. Lane D.J., Pace B., Olsen G.J., Stahl D.A., Sogin M.L., Pace N.R. ( 1985;). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82 69556959 [View Article] [PubMed].
    [Google Scholar]
  13. Logan N.A., Berge O., Bishop A.H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 21142121 [View Article] [PubMed].
    [Google Scholar]
  14. Madhaiyan M., Poonguzhali S., Lee J.-S., Lee K.-C., Hari K. ( 2011;). Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. Antonie van Leeuwenhoek 100 437444 [View Article] [PubMed].
    [Google Scholar]
  15. Manickam N., Singh N.K., Bajaj A., Kumar R.M., Kaur G., Kaur N., Bala M., Kumar A., Mayilraj S. ( 2014;). Bacillus mesophilum sp. nov., strain IITR-54T, a novel 4-chlorobiphenyl dechlorinating bacterium. Arch Microbiol 196 517523 [View Article] [PubMed].
    [Google Scholar]
  16. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  17. McKerrow J., Vagg S., McKinney T., Seviour E.M., Maszenan A.M., Brooks P., Seviour R.J. ( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30 178182 [View Article] [PubMed].
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  19. Priest F.G., Goodfellow M., Todd C. ( 1988;). A numerical classification of the genus Bacillus . J Gen Microbiol 134 18471882 [PubMed].
    [Google Scholar]
  20. Reddy S.V., Thirumala M., Farooq M., Sasikala C., Ramana C.V. ( 2015;). Bacillus lonarensis sp. nov., an alkalitolerant bacterium isolated from a soda lake. Arch Microbiol 197 2734 [View Article] [PubMed].
    [Google Scholar]
  21. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  22. Schleifer K.H. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18 123156 [View Article].
    [Google Scholar]
  23. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  24. Seldin L., Dubnau D. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35 151154 [View Article].
    [Google Scholar]
  25. Sonalkar V.V., Mawlankar R., Venkata Ramana V., Joseph N., Shouche Y.S., Dastager S.G. ( 2015;). Bacillus filamentosus sp. nov., isolated from sediment sample. Antonie van Leeuwenhoek 107 433441 [View Article] [PubMed].
    [Google Scholar]
  26. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch.V. ( 2013;). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 63 21322137 [View Article] [PubMed].
    [Google Scholar]
  27. Subhash Y., Sasikala Ch., Ramana Ch.V. ( 2014;). Bacillus luteus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64 15801586 [View Article] [PubMed].
    [Google Scholar]
  28. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  29. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. ( 2003;). Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 53 725730 [View Article] [PubMed].
    [Google Scholar]
  30. Zhai L., Liao T., Xue Y., Ma Y. ( 2012;). Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake. Int J Syst Evol Microbiol 62 949953 [View Article] [PubMed].
    [Google Scholar]
  31. Zhang J., Wang J., Fang C., Song F., Xin Y., Qu L., Ding K. ( 2010;). Bacillus oceanisediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 60 29242929 [View Article] [PubMed].
    [Google Scholar]
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