1887

Abstract

Two independent surveys of yeasts associated with different bromeliads in different Brazilian regions led to the proposal of a novel yeast species, sp. nov., belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences in the internal transcribed spacer (ITS) region and D1/D2 domain of the LSU rRNA gene suggested affinity to a phylogenetic lineage that includes and . Six isolates of the novel species were obtained from different bromeliads and regions in Brazil. Sequence analysis of the D1/D2 domains of the large subunit of the rRNA gene showed that the novel species differs from . and by 85 and 64 nt substitutions, respectively and by more than 75 nt substitutions in the ITS region. Phenotypically, sp. nov. can be distinguished from both species based on the assimilation of -erythritol, which was negative for sp. nov. but positive for the others, assimilation of -glucosamine, which was positive for sp. nov. but negative for and of -sorbose, which was negative for sp. nov. but positive for . The novel species sp. nov. is proposed to accommodate these isolates. The type strain of sp. nov. is UFMG-CM-Y379 (BRO443; ex-type CBS 13870).

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2015-08-01
2019-10-23
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References

  1. Altschul S.F. , Gish W. , Miller W. , Myers E.W. , Lipman D.J. . ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Araújo F.V. , Rosa C.A. , Freitas L.F.D. , Lachance M.A. , Vaughan-Martini A. , Mendonça-Hagler L.C. , Hagler A.N. . ( 2012;). Kazachstania bromeliacearum sp. nov., a yeast species from water tanks of bromeliads. Int J Syst Evol Microbiol 62: 1002–1006 [CrossRef] [PubMed].
    [Google Scholar]
  3. Boekhout T. , Fonseca A. , Sampaio J.P. , Bandoni R.J. , Fell J.W. , Kwon-Chung K.J. . ( 2011a;). Discussion of teleomorphic and anamorphic basidiomycetous yeasts. . In The Yeasts, a Taxonomic Study , 5th edn. vol. 3, pp. 1339–1372. Edited by Kurtzman C. P. , Fell J. W. , Boekhout T. . Amsterdam: Elsevier; [CrossRef].
    [Google Scholar]
  4. Boekhout T. , Bai F.Y. , Nakase T. . ( 2011b;). Bullera Derx (1930). . In The Yeasts, a Taxonomic Study , 5th edn. vol. 3, pp. 1623–1659. Edited by Kurtzman C. P. , Fell J. W. , Boekhout T. . Amsterdam: Elsevier; [CrossRef].
    [Google Scholar]
  5. Fell J.W. , Boekhout T. , Fonseca A. , Scorzetti G. , Statzell-Tallman A. . ( 2000;). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50: 1351–1371 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Fungsin B. , Takashima M. , Artjariyasripong S. , Potacharoen W. , Nakase T. . ( 2003;). Bullera sakaeratica sp. nov., a new species of ballistoconidium-forming yeast found in Thailand. Microbiol Cult Collect 19: 33–39.
    [Google Scholar]
  8. Inácio J. , Landell M.F. , Valente P. , Wang P.H. , Wang Y.T. , Yang S.H. , Manson J.S. , Lachance M.A. , Rosa C.A. , Fonseca A. . ( 2008;). Farysizyma gen. nov., an anamorphic genus in the Ustilaginales to accommodate three novel epiphytic basidiomycetous yeast species from America, Europe and Asia. FEMS Yeast Res 8: 499–508 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kurtzman C.P. , Robnett C.J. . ( 1998;). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73: 331–371 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kurtzman C.P. , Fell F.W. , Boekhout T. , Robert V. . ( 2011;). Methods for isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts, a Taxonomic Study , 5th edn. vol. 1, pp. 87–110. Edited by Kurtzman C. P. , Fell J. W. , Boekhout T. . Amsterdam: Elsevier; [CrossRef].
    [Google Scholar]
  12. Lachance M.A. , Bowles J.M. , Starmer W.T. , Barker J.S.F. . ( 1999;). Kodamaea kakaduensis Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45: 172–177 [CrossRef] [PubMed].
    [Google Scholar]
  13. Landell M.F. , Inácio J. , Fonseca A. , Vainstein M.H. , Valente P. . ( 2009;). Cryptococcus bromeliarum sp. nov., an orange-coloured basidiomycetous yeast isolated from bromeliads in Brazil. Int J Syst Evol Microbiol 59: 910–913 [CrossRef] [PubMed].
    [Google Scholar]
  14. Landell M.F. , Billodre R. , Ramos J.P. , Leoncini O. , Vainstein M.H. , Valente P. . ( 2010;). Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil. Int J Syst Evol Microbiol 60: 244–248 [CrossRef] [PubMed].
    [Google Scholar]
  15. Landell M.F. , Brandão L.R. , Barbosa A.C. , Ramos J.P. , Safar S.V. , Gomes F.C.O. , Sousa F.M. , Morais P.B. , Broetto L. , other authors . ( 2014;). Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil. Int J Syst Evol Microbiol 64: 1970–1977 [CrossRef] [PubMed].
    [Google Scholar]
  16. Miller M.A. , Pfeiffer W. , Schwartz T. . ( 2010;). Creating the CIPRES Science Gateway for inference of large phylogenetic trees. . In Proceedings of the Gateway Computing Environments Workshop (GCE) 14 Nov. 2010 1–8 New Orleans, LA:.
    [Google Scholar]
  17. Nakase T. . ( 2000;). Expanding world of ballistosporous yeasts: distribution in the phyllosphere, systematics and phylogeny. J Gen Appl Microbiol 46: 189–216 [CrossRef] [PubMed].
    [Google Scholar]
  18. Nakase N. , Itoh M. , Takematsu A. , Bandoni R.J. . ( 1990;). Bullera miyagiana, a new species of ballistospore-forming yeast isolated from Abies firma collected in Japan. J Gen Appl Microbiol 36: 33–39 [CrossRef].
    [Google Scholar]
  19. O'Donnell K. . ( 1993;). Fusarium and its near relatives. . In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Systematics, pp. 225–233. Edited by Reynolds D. R. , Taylor J. W. . Wallingford: CAB International;.
    [Google Scholar]
  20. Péter G. , Tornai-Lehoczki J. , Dlauchy D. . ( 2009;). Candida ogatae sp. nov., an anamorphic member of the Kuraishia clade. FEMS Yeast Res 9: 328–333 [CrossRef] [PubMed].
    [Google Scholar]
  21. Posada D. , Buckley T.R. . ( 2004;). Model selection and model averaging in phylogenetics: advantages of akaike information criterion and bayesian approaches over likelihood ratio tests. Syst Biol 53: 793–808 [CrossRef] [PubMed].
    [Google Scholar]
  22. Rambaut A. , Suchard M.A. , Xie D. , Drummond A.J. . ( 2014;). Tracer v1.6. Available from http://beast.bio.ed.ac.uk/Tracer .
  23. Ramos J.P. , Valente P. , de Souza R.A. , Rosa C.A. , Leoncini O. . ( 2001;). Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. Lett Appl Microbiol 33: 206–210 [CrossRef] [PubMed].
    [Google Scholar]
  24. Rambaut A. . ( 2009;). FigTree v1.2.3. Institute of Evolutionary Biology, Univ. of Edinburgh. Available at: http://tree.bio.ed.ac.uk/software/figtree..
  25. Rannala B. , Yang Z. . ( 1996;). Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J Mol Evol 43: 304–311 [CrossRef] [PubMed].
    [Google Scholar]
  26. Rodríguez F. , Oliver J.L. , Marín A. , Medina J.R. . ( 1990;). The general stochastic model of nucleotide substitution. J Theor Biol 142: 485–501 [CrossRef] [PubMed].
    [Google Scholar]
  27. Ronquist F. , Teslenko M. , van der Mark P. , Ayres D.L. , Darling A. , Höhna S. , Larget B. , Liu L. , Suchard M.A. , Huelsenbeck J.P. . ( 2012;). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61: 539–542 [CrossRef] [PubMed].
    [Google Scholar]
  28. Ruivo C.C.C. , Lachance M.A. , Rosa C.A. , Bacci M. Jr , Pagnocca F.C. . ( 2005;). Candida bromeliacearum sp. nov. and Candida ubatubensis sp. nov., two yeast species isolated from the water tanks of Canistropsis seidelii (Bromeliaceae). Int J Syst Evol Microbiol 55: 2213–2217 [CrossRef] [PubMed].
    [Google Scholar]
  29. Safar S.V.B. , Gomes F.C.O. , Marques A.R. , Lachance M.A. , Rosa C.A. . ( 2013;). Kazachstania rupicola sp. nov., a yeast species isolated from water tanks of a bromeliad in Brazil. Int J Syst Evol Microbiol 63: 1165–1168 [CrossRef] [PubMed].
    [Google Scholar]
  30. Sousa F.M.P. , Morais P.B. , Lachance M.A. , Rosa C.A. . ( 2014;). Hagleromyces gen. nov., a yeast genus in the Saccharomycetaceae, and description of Hagleromyces aurorensis sp. nov., isolated from water tanks of bromeliads. Int J Syst Evol Microbiol 64: 2915–2918 [CrossRef] [PubMed].
    [Google Scholar]
  31. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  32. Wang Q.M. , Bai F.Y. . ( 2008;). Molecular phylogeny of basidiomycetous yeasts in the Cryptococcus luteolus lineage (Tremellales) based on nuclear rRNA and mitochondrial cytochrome b gene sequence analyses: proposal of Derxomyces gen. nov. and Hannaella gen. nov., and description of eight novel Derxomyces species. FEMS Yeast Res 8: 799–814.[CrossRef]
    [Google Scholar]
  33. Wang Q.-M. , Bai F.-Y. , Fungsin B. , Boekhout T. , Nakase T. . ( 2011;). Proposal of Mingxiaea gen. nov. for the anamorphic basidiomycetous yeast species in the Bulleribasidium clade (Tremellales) based on molecular phylogenetic analysis, with six new combinations and four novel species. Int J Syst Evol Microbiol 61: 210–219.[CrossRef]
    [Google Scholar]
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