1887

Abstract

Using a polyphasic taxonomic approach, we investigated three bacterial strains – IWT30, IWT8 and IWT75 – isolated from total mixed ration silage prepared in Hachimantai, Iwate, Japan. The isolates comprised Gram-stain positive, non-motile, non-spore-forming, catalase-negative, rod-shaped bacteria. Good growth occurred at 15–45 °C and at pH 4.0–7.5. Their major cellular fatty acids were Cω9 and C cyclo 9,10.The G+C content of genomic DNA of strain IWT30 was 44.6 mol%. Comparative 16S rRNA gene sequence analysis showed that these novel strains belonged to the genus These strains shared 100 % 16S rRNA gene sequence similarity and were most closely related to the type strains of , , , , and , with sequence similarity values of 99.5, 98.8, 98.7, 97.8, 97.8 and 96.8 %, respectively. The level of DNA–DNA relatedness between these strains and their closest phylogenetic neighbours was less than 30 %. On the basis of additional phylogenetic analysis of and gene sequences and phenotypic and chemotaxonomic characteristics, we conclude that these three strains represent a novel species of the genus , for which we propose the name sp. nov. The type strain is IWT30 ( = JCM 19805 = DSM 28580).

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2015-06-01
2019-10-21
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